葡萄ZTL、COP1基因的鉴定及其在不同光质和转光条件下的表达分析

发布时间：2018-03-05 13:11

本文选题：葡萄试管苗　切入点：ZTL基因　出处：《果树学报》2017年05期 　论文类型：期刊论文

【摘要】：【目的】在葡萄试管苗离体培养下,研究不同波长对基因ZTL、COP1表达的影响。【方法】以‘红地球’葡萄(Vitis vinifera‘Red Globe’)试管苗为材料,经RT-PCR法克隆获得葡萄ZTL、COP1基因全长cDNA,并利用生物信息学分析了以上基因的理化性质、蛋白质二级结构、亚细胞定位以及系统进化树。利用q RT-PCR技术分析了以上基因在不同光质处理下表达量的变化情况。利用荧光参数系统分析不同光质对葡萄试管苗生长的影响。【结果】ZTL的分子式为C1972H3107N553O576S20,是亲水性稳定蛋白,脂溶性较差;COP1的分子式为C832H1291N209O225S9,是疏水性稳定蛋白,脂溶性较好。ZTL和COP1的二级结构均由α-螺旋、β-转角、无规则卷曲和延伸链结构组成;亚细胞定位分析结果显示,ZTL、COP1均位于细胞质中;系统进化分析表明,ZTL与桃的亲缘关系最近,COP1与其他物种的亲缘关系均较远,单独聚为一个亚族。在不同光质处理后ZTL均为上调表达,其中白光转蓝光、红光、红光转白光、红光转蓝光和蓝光转红光处理后分别上调达11.2、22.3、12.0、32.9和19.6倍。不同光质处理后COP1基因的表达呈现不同的变化趋势:红光转蓝光处理后,COP1上调1.97倍,红光处理后表达量无显著变化,其他光质处理后均为下调表达,其中蓝光转白光和蓝光转红光处理后下调达18倍。荧光参数分析表明,qP、qN、NPQ和F_v/F_m在转光培养中表达活跃,均在红光转蓝光的转光培养下表达量最高。【结论】ZTL在葡萄转光培养中表达活跃,均呈显著上调趋势,而COP1总体表现为下调趋势。
[Abstract]:[objective] to study the effect of different wavelengths on the expression of gene ZTLCOP1 in vitro culture of grape plantlets. [methods] Vitis vinifera'Red vinifera'Red Globeb seedlings of 'Red Earth' were used as materials. The full-length cDNAs of Grape ZTL-COP1 gene were cloned by RT-PCR method. The physicochemical properties and protein secondary structure of the genes were analyzed by bioinformatics. Subcellular localization and phylogenetic tree. Q RT-PCR technique was used to analyze the expression changes of the above genes under different light quality treatments. Fluorescence parameters were used to analyze the effects of different light quality on the growth of grape plantlets in vitro. The molecular formula of ZTL is C1972H3107N553O576S20, which is a hydrophilic stable protein. The molecular formula of C832H1291N209O225S9 is hydrophobic stable protein. The secondary structures of liposoluble. ZTL and COP1 are composed of 伪 -helix, 尾 -rotation, irregular curl and extended chain structure, and the subcellular localization analysis shows that ZTL COP1 is located in the cytoplasm. Phylogenetic analysis showed that the phylogenetic relationship between ZTLs and other species was far away, and ZTL was expressed up-regulated after different light quality treatments, including white light to blue light, red light to white light, red light to white light, to white light to white light, and to white light, to white light, to white light to white light, to white light to blue light, to red light to white light, to white light to white light. After red light to blue light and blue light to red light, the expression of COP1 gene increased by 11.2222.312.0 and 19.6 times, respectively. The expression of COP1 gene showed a different trend after different light quality treatments: the expression of COP1 gene increased by 1.97 times after the treatment of red light to blue light, but there was no significant change after the treatment of red light to blue light. Other light quality treatments were all down-regulated, of which the blue light to white light and the blue light to red light were down regulated by 18 times. The fluorescence parameter analysis showed that the NPQ and FSP / FSP were active in the light conversion culture. [conclusion] the expression of ZTL was active and up-regulated, but COP1 was down-regulated in general. [conclusion] the expression of ZTL was significantly up-regulated in grape under light conversion to blue light. [conclusion] the expression of ZTL was active in grape under light conversion and showed a significant up-regulation trend, while COP1 showed a down-regulation trend.
【作者单位】：甘肃农业大学园艺学院;
【基金】：国家自然科学基金(31401500)
【分类号】：S663.1

【相似文献】