棉花CBF基因的克隆及其转基因烟草的抗寒性分析(2)

  本文关键词：棉花CBF基因的克隆及其转基因烟草的抗寒性分析，由笔耕文化传播整理发布。

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作 物 学 报 第37卷

的烟草受冷处理后, 游离脯氨酸和可溶性糖的含量都比野生型有所提高。这说明GbCBF1的表达能够提高植物对于低温的耐受性。另外, 本研究中NOS:GbCBF1转基因烟草在0℃冷处理前后, 可溶性糖含量均大大高于35S:GbCBF1转基因烟草, 而在0℃处理时的电解质渗漏率与35S:GbCBF1转基因烟草却没有明显差异, 说明在转基因烟草中可溶性糖含量的提高并不是植物耐寒性增强的主要原因, 这与Cholewa等[25]

和Wanner等

[26]

的研究结果一致。

可溶性糖含量与植物耐寒性之间的关系, 需要进一

步的研究。

4 结论

从棉花品种中棉12、中棉36和海岛棉7124中克隆并鉴定了CBF全长基因。CBF在棉花中以多拷贝的形式存在。GbCBF1基因的表达受低温、ABA、干旱和盐胁迫等多种逆境信号的诱导。获得了转GbCBF1基因的烟草, 从而提高了烟草的耐寒性。

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  本文关键词：棉花CBF基因的克隆及其转基因烟草的抗寒性分析，由笔耕文化传播整理发布。