牛IRS1基因3’UTR的克

发布时间：2018-03-07 17:06

  本文选题：牛　切入点：胰岛素受体基质　出处：《河南农业大学学报》2017年01期 　论文类型：期刊论文

【摘要】：以奶牛乳腺组织为材料,采用3’RACE技术克隆到奶牛IRS1基因3’UTR的全长,进一步对测得的序列进行了验证,同时利用Target Scan 7.0和Pic Tar软件预测可能结合的microRNA,并对多个软件预测结合的microRNA,进行最小结合自由能分析。结果表明,成功克隆了1 327 bp的牛IRS1 3’UTR全长,与NCBI上的预测序列一致率达到99%;miR-128、miR-96、miR-27a为2个软件共同预测到的结合microRNA;3个结合位点的最小自由能分别为-89.58、-87.91、-100.88 k J·mol-1,表明牛IRS1基因表达可能受到这3个microRNA的调控。
[Abstract]:The full-length IRS1 gene 3UTR of dairy cattle was cloned by using 3race technique from mammary tissue of dairy cattle, and the sequence was further verified. At the same time, Target Scan 7.0 and Pic Tar software were used to predict the possible binding microRNAs, and the minimum binding free energy of multiple microRNAs was analyzed. The results showed that 1 327 BP bovine IRS1 3 UTR was cloned successfully. The coincident rate of the predicted sequence with the predicted sequence on NCBI was 99%, and the minimum free energy of the three binding sites was -89.58 ~ 87.91 ~ 100.88 kJ 路mol ~ (-1), respectively, indicating that the expression of bovine IRS1 gene might be regulated by these three kinds of microRNA, and the minimum free energy of the three binding sites was -89.58% -87.91 ~ (-1) -100.88 kJ 路mol ~ (-1), respectively, which indicated that the expression of bovine IRS1 gene might be regulated by these three microRNA.
【作者单位】： 河南农业大学生命科学学院;河南省农业科学院畜牧兽医研究所;
【基金】：国家自然科学基金项目(31501978) 河南省高等学校重点科研项目(16A230010)
【分类号】：S823
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本文编号：1580182