3株H1N1亚型猪流感病毒的HA基因遗传信息及抗原特异性分析

发布时间：2018-03-08 05:11

本文选题：猪流感病毒　切入点：HN　出处：《华南农业大学学报》2017年05期 　论文类型：期刊论文

【摘要】：【目的】分析3株属于欧亚类禽(SWSS1)、经典(SWL6)与Pdm09H1N1(Pdm091057)分支的猪流感病毒的HA基因遗传信息及抗原的特异性,为HA基因抗原表位的功能研究及流感防控奠定基础。【方法】以SWSS1株、SWL6株和Pdm091057株流感病毒为材料,比较分析3株H1N1亚型HA基因片段的遗传信息,制备全病毒灭活疫苗,各免疫3只雌性新西兰大白兔,采用血凝抑制(Hemagglutination inhibition,HI)反应试验检测抗体滴度。【结果】3株病毒的HA基因片段的氨基酸序列相似性为69.4%~89.1%,各分支的HA基因的抗原表位存在差异;3株病毒经过2次免疫后平均HI抗体滴度均能达1 280以上,且SWSS1的平均HI抗体滴度高达2 560。同时SWSS1与SWL6、Pdm091057这2株病毒均无血清学交叉反应,而SWL6与Pdm091057有较低的血清学交叉反应。【结论】3株猪流感病毒的HA基因片段抗原表位存在着差异,可能是3毒株之间血清学交叉反应较低的原因。3株病毒免疫原性均较好,可作为候选疫苗株。
[Abstract]:[objective] to analyze the genetic information of HA gene and the specificity of antigens of three strains of swine influenza virus belonging to Eurasian avian species SWSS _ 1 (classic SWL6) and Pdm09H1 / Pdm091057). [methods] using SWSS1 strain SWL6 strain and Pdm091057 strain of influenza virus as materials, the genetic information of three H1N1 subtype HA gene fragments were compared and analyzed, and the whole virus inactivated vaccine was prepared. Three female New Zealand white rabbits were immunized. Hemagglutination inhibition inhibition (HI) assay was used to detect antibody titer. [results] the amino acid sequence similarity of HA gene fragments of the three strains was 69.4 and 89.1.The antigenic epitopes of HA gene in each branch were different. The titers of HI antibody were above 1 280. The average HI antibody titer of SWSS1 was as high as 2560. At the same time, there was no serological cross reaction between SWSS1 and SWL6 Pdm091057 strain, while SWL6 and Pdm091057 had lower serological cross reaction. [conclusion] the HA gene fragment antigen epitopes of three swine influenza viruses were different. It may be the reason that the serological cross reaction among the three strains is low. 3 strain is good in immunogenicity and can be used as a candidate vaccine strain.
【作者单位】：广东省动物源性人兽共患病预防与控制重点实验室/国家生猪种业工程技术研究中心/华南农业大学兽医学院;华南农业大学医院;烟台市福山区动物卫生监督所;
【基金】：国家重点研发计划(2016YFD0500707) 国家生猪产业技术体系专项(CARS-36) 广东省科技计划(2013B020202002) 广东省条财处项目(2012B060400015) 广东省高等学校优秀青年教师培养计划(YQ201530)
【分类号】：S852.651

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