长春花生物碱合成途径相关基因表达丰度与MeJA处理后基因表达差异分析

发布时间：2018-03-08 06:32

本文选题：长春花萜类吲哚生物碱　切入点：合成途径　出处：《基因组学与应用生物学》2017年03期 　论文类型：期刊论文

【摘要】：长春花(Catharanthus roseus)中含有丰富的次生代谢产物,可产生130多种萜类吲哚生物碱,包括目前临床应用最广的天然植物抗癌药物长春碱(vinblastine)和长春新碱(vincristine)。长春花萜类吲哚生物碱(terpenoid indole alkaloids,TIAs)合成途径包括在内韧皮部相关薄壁细胞、上皮细胞、异细胞和乳管细胞等多种组织细胞内进行的20多步酶促反应。本研究选取TIAs途径上的CPR、LAMT、SLS、STR、SGD、TDC、16-OMT、T16H、D4H等9个酶基因,基于长春花转录组数据库,分析其在各组织器官和MeJA处理下的无菌苗、毛状根和悬浮细胞中的数字表达谱,找出其中可能对MeJA信号响应的基因。本研究发现,MeJA处理后,长春花无菌苗中以上9个基因表达量均发生上调;毛状根中除了TDC,另外8个基因表达丰度均为上调;悬浮细胞中除了D4H,另外8个基因表达丰度均上调。对整个转录组数据进行分析,发现经过MeJA处理后,仅有673条Unigene能在无菌苗、毛状根和悬浮细胞中均表现出差异表达,说明无菌苗、毛状根和悬浮细胞的MeJA应答机制存在较大不同。对这些能在3种体系中均发生差异表达的基因进行功能分类,主要包括初生代谢相关蛋白、次生代谢相关蛋白、植物激素相关蛋白、矿质离子结合蛋白、转运蛋白、信号转导相关蛋白、细胞壁合成与降解相关蛋白、转录因子、植物逆境响应蛋白9个方面。本研究可为通过外源因子调控长春花生物碱的生物合成提供参考。
[Abstract]:Catharanthus roseus, which contains abundant secondary metabolites, can produce more than 130 kinds of terpenoid indole alkaloids. Including vinblastine (vinblastine) and vinblastine (vinblastine) and vincristine (vinblastine), the synthetic pathway of terpenoid indole alkaloidsof vinblastine (vinblastine) and vinblastine (vinblastine), including phloem related parenchyma cells, epithelial cells, More than 20 steps of enzymatic reactions were carried out in various tissue cells such as xenoblasts and lactobacillus cells. In this study, nine enzyme genes, such as TIAs pathway, were selected to analyze the aseptic vaccine treated with MeJA and tissue organs, based on the transcriptome database of roseus roseus, and nine enzyme genes, such as TDCC16-OMTT1-T16HD4H, were selected. The digital expression profiles in hairy roots and suspension cells were used to identify the genes that might respond to MeJA signal. In this study, it was found that the above nine genes were up-regulated in aseptic seedlings of Catharanthus roseus after treatment with Meja. In hairy roots, except TDC, the other 8 genes were up-regulated, and in suspension cells, except D4H, the other eight genes were up-regulated. By analyzing the whole transcriptional group data, it was found that only 673 Unigene could be expressed in sterile vaccine after MeJA treatment. The differential expression in hairy root and suspension cell indicated that the mechanism of MeJA response in sterile vaccine, hairy root and suspension cell was different. These proteins include primary metabolism-associated proteins, secondary metabolization-related proteins, plant hormone related proteins, mineral ion binding proteins, transporter proteins, signal transduction related proteins, cell wall synthesis and degradation related proteins, transcription factors, This study can be used as a reference for regulating the biosynthesis of alkaloids in roseus roseus by exogenous factors.
【作者单位】：西南大学生命科学学院;西南大学—西藏农牧学院药用植物联合研发中心;重庆科技学院;
【基金】：国家级大学生创新创业训练计划项目(201510635011) 国家863计划(2011AA100605) 新世纪优秀人才支持计划(NCET-12-0930)项目基金共同资助
【分类号】：Q943.2

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