甘草水提物中miRNA对人免疫细胞基因表达的影响

发布时间：2018-03-08 18:37

本文选题：甘草水提物　切入点：小分子RNA　出处：《中国中药杂志》2017年09期 　论文类型：期刊论文

【摘要】：微小RNA(micro RNA,miRNA)是一类在转录后水平调节基因表达的非编码小分子RNA,其调控基因表达、影响细胞活性的功能已为人们所重视。近年来miRNA的跨界调控成为新的研究方向,为人们更全面地认识和了解miRNA的功能提供了新的视角。植物miRNA由于通常经过了甲基化修饰,因此较为稳定,不易降解。前期研究表明,甘草干燥药材的水煎剂中存在着丰富的小分子RNA,这为了解甘草的作用机制提供了新的思路。在本研究中,利用从甘草水煎剂中提取的小分子RNA以及合成的miRNA模拟物(mimic)作用于分离自健康人的外周血单核细胞(peripheral blood mononuclear cell,PBMC),然后利用RT-PCR对其模式识别受体(PRR)基因以及部分转录因子、信号分子和细胞因子的基因表达变化进行了分析。结果表明甘草miRNA对人免疫细胞的基因表达具有明显的调节作用,能够显著抑制T细胞分化相关基因的表达,以及炎症和凋亡相关基因的表达。这为进一步更全面地了解甘草的作用机制以及中药新药的研制带来了新的思路。
[Abstract]:Small RNA(micro RNAs are a kind of non-coding small molecule RNAs that regulate gene expression at post-transcriptional level. The function of regulating gene expression and affecting cell activity has been paid attention to. Recently, the cross-border regulation of miRNA has become a new research direction. It provides a new perspective for a more comprehensive understanding and understanding of the functions of miRNA. Plant miRNA is stable and difficult to degrade because it is usually modified by methylation. There are abundant small molecule RNAs in the decoction of licorice dried herbs, which provides a new idea for understanding the mechanism of licorice. RNA, a small molecule extracted from Glycyrrhiza uralensis decoction, and mimicoid, a synthetic miRNA mimicoid, were used to treat peripheral blood monocytes isolated from healthy individuals, and then RT-PCR was used to identify its pattern recognition receptor (PRRR) gene and some transcription factors. The changes of gene expression of signal molecules and cytokines were analyzed. The results showed that Glycyrrhiza uralensis (miRNA) could significantly regulate the gene expression of human immune cells and inhibit the expression of genes related to T cell differentiation. And the expression of inflammatory and apoptosis-related genes, which provides a new idea for the further understanding of the mechanism of licorice and the development of new drugs of traditional Chinese medicine.
【作者单位】：广东药科大学生命科学与生物制药学院广东省生物技术候选药物研究重点实验室;
【基金】：国家自然科学基金项目(31100664,31300737,81303292) 广东省自然科学基金项目(10151022401000024,2014A030313586) 广东省科技计划项目(2016A020215157)
【分类号】：R285

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