蛋源大肠埃希菌的分离鉴定及毒力基因和耐药基因检测

发布时间：2018-03-09 19:50

本文选题：蛋源大肠埃希菌　切入点：毒力基因　出处：《动物医学进展》2017年02期 　论文类型：期刊论文

【摘要】：为了调查鸡蛋中大肠埃希菌的感染情况,为生产实践提供参考,从昆明市区鸡蛋中分离出了3株大肠埃希菌,并对其毒力基因、耐药基因、动物致病性及药物敏感性进行了研究。结果表明,菌株KM1携带的毒力基因检出率75%,耐药基因检出率77.3%;菌株KM2携带的毒力基因和耐药基因检出率居中;菌株KM3毒力基因检出率91.7%,耐药性基因检出率50%。动物毒力检测结果 KM1为不致病菌株,KM2为低致病菌株,KM3为中度致病菌株。药敏试验结果显示,分离菌株对头孢噻肟、头孢曲松钠敏感,对庆大霉素、氨苄西林等具有不同程度的耐药性。中草药对3株大肠埃希菌的抑制效果各有所不同,相比之下连翘与松针的效果较好。本试验为研究昆明市区鸡蛋中大肠埃希菌污染与防控提供了理论依据。
[Abstract]:In order to investigate the infection of Escherichia coli in eggs and provide reference for production practice, three strains of Escherichia coli were isolated from eggs in Kunming city, and their virulence genes and drug resistance genes were analyzed. Animal pathogenicity and drug sensitivity were studied. The results showed that the detection rate of virulence gene and drug resistance gene of strain KM1 was 75 and 77.3 respectively, the detection rate of virulence gene and drug resistance gene of strain KM2 was the middle. The detection rate of KM3 virulence gene and drug resistance gene were 91.7 and 50.The results of animal virulence test showed that KM1 was non-pathogenic strain, KM2 strain was low pathogenic strain, KM3 strain was moderate pathogenic strain, and the drug sensitivity test showed that the isolated strain was resistant to cefotaxime. Ceftriaxone sodium was sensitive and resistant to gentamicin and ampicillin to varying degrees. The inhibitory effects of Chinese herbal medicine on three strains of Escherichia coli were different. Compared with forsythia forsythia and pine needle, this experiment provides a theoretical basis for studying the contamination and control of Escherichia coli in eggs in Kunming city.
【作者单位】：西南林业大学生命科学学院;
【基金】：云南省优势特色重点学科生物学建设项目(50097505) 云南省高校林下生物资源保护及利用科技创新团队(51400605)
【分类号】：S852.61

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