基于肿瘤基因作用的妊娠妇女调节性免疫细胞与巨细胞病毒的相关性研究

发布时间：2018-03-09 23:10

本文选题：妊娠　切入点：巨细胞病毒(CMV)　出处：《病毒学报》2017年04期 　论文类型：期刊论文

【摘要】：研究基于肿瘤基因作用的妊娠妇女调节性免疫细胞与巨细胞病毒的相关性。收集2015年5月~2016年9月四川省人民医院就诊的妊娠患者,病例纳入标准:年龄范围在22~40岁;初次妊娠患者;排除前置胎盘、先兆流产和妊娠高血压等异常妊娠患者;排除合并慢阻肺、糖尿病、艾滋病等慢性疾病患者;排除艾滋病和乙肝病毒等感染患者;排除长期吸烟、酗酒患者。梯度离心的方法分离患者外周血的淋巴细胞。CD4、CD25和Foxp3流式抗体标记调节性T细胞(Treg细胞);CD19和TGF~-β流式抗体标记调节性B细胞(Breg细胞);CD4、CXCR5和Foxp3流式抗体标记滤泡调节性T细胞(Tfr细胞)。结果表明,对照组共有30例正常妊娠患者,年龄在22岁至37岁之间;CMV感染组包括30名CMV感染的妊娠患者,年龄在22岁至39岁之间。CMV感染组中CD4~+CD25~+Foxp3~+的Treg细胞、CD19~+TGF~-β~+的Breg细胞和CD4~+CXCR5~+Foxp3~+的Tfr细胞比例均显著的低于对照组,分别下降了31.9%、78.5%和65.1%,结果具有统计学差异(P0.05)。CMV感染后,妊娠患者体内Treg细胞、Breg细胞和Tfr细胞等调节性免疫细胞的比例均显著下降,可能对胎儿的生长发育造成影响。
[Abstract]:To study the relationship between regulatory immune cells and cytomegalovirus (CMV) in pregnant women based on tumor gene function. From May 2015 to September 2016 pregnancy patients in Sichuan Provincial people's Hospital were collected. The inclusion criteria were as follows: the age range was 22 ~ 40 years old; First pregnancy; exclusion of placenta previa, threatened abortion, pregnancy hypertension and other abnormal pregnancy patients; exclusion of patients with chronic diseases such as chronic obstructive pulmonary disease, diabetes, AIDS; exclusion of AIDS and hepatitis B virus infection patients; Excluding long-term smoking, Alcoholic patients. Separation of peripheral blood lymphocytes. CD4, CD25 and Foxp3 flow antibody labeled regulatory T cell / Treg cells, CD19 and TGF- 尾 flow antibody labeled regulatory B cells by gradient centrifugation with CD4CXCR5 and Foxp3 flow antibody labeled filter. The results showed that, There were 30 normal pregnant women in the control group and 30 pregnant women with CMV infection between 22 and 37 years of age. The percentage of CD19 ~ TGF- 尾 and CD4 ~ CXCR5 ~ Foxp3 ~ Tfr cells in Treg cells of CD4 ~ CD25 ~ Foxp3~ and CD4 ~ CXCR5 ~ Foxp3 ~ were significantly lower than those in control group, and the percentage of CD4- CD25 ~ Foxp3- 尾 and CD4CXCR5 ~ Foxp3- 尾 cells were significantly lower than those in control group, which decreased by 31.98.5% and 65.1%, respectively. The results showed that there were significant differences in the percentage of CD19 ~ TGF- 尾 and CD4 ~ CXCR5 ~ Foxp3- 尾 cells between the two groups. The percentage of regulatory immune cells such as Treg cells and Tfr cells in pregnant women decreased significantly, which may affect the growth and development of fetuses.
【作者单位】：四川省人民医院妇产科;四川省人民医院药学部;
【基金】：国家自然科学基金(项目号:81301746),题目:新基因C16orf72在肿瘤发生中的作用及分子机制~~
【分类号】：R714.251

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