紫花苜蓿的抗菌肽基因Rev4遗传转化

发布时间：2018-03-10 09:27

本文选题：紫花苜蓿　切入点：抗菌肽　出处：《江苏农业科学》2017年07期 　论文类型：期刊论文

【摘要】：抗菌肽是机体先天免疫的重要组成成分,是许多生物体抵抗外来致病菌侵袭的第1道屏障。Indolicidin是一个13氨基酸残基肽,从牛的嗜中性粒细胞的胞浆小颗粒分离而来,具有广谱抗菌性,Rev4是其人工设计的反向序列。利用经典叶盘法,以根癌农杆菌A.GV3850介导紫花苜蓿遗传转化,GUS瞬时表达检测转化体系建立成功;压力筛选后叶片基因组DNA的PCR成功检测到抗菌肽基因条带,PCR产物回收测序得到13株转化紫花苜蓿。
[Abstract]:Antimicrobial peptide is an important component of innate immunity. It is the first barrier for many organisms to resist the invasion of foreign pathogens. Indolicidin is a 13-amino acid peptide isolated from the cytoplasmic granules of bovine neutrophils. The reverse sequence with broad spectrum antimicrobial activity was established successfully by Agrobacterium tumefaciens A. GV3850 mediated genetic transformation of alfalfa. After pressure screening, 13 transformed alfalfa strains were successfully detected by PCR from genomic DNA of leaves, and 13 strains of alfalfa were recovered and sequenced.
【作者单位】：吉林师范大学植物资源科学与绿色生产吉林省重点实验室;
【基金】：国家自然科学基金(编号:31070224)
【分类号】：S541.9

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