紫苏种子脂肪酸代谢及关键酶基因调控油脂合成规律的研究

发布时间：2018-03-10 15:03

本文选题：紫苏　切入点：种子油脂　出处：《中国粮油学报》2016年03期 　论文类型：期刊论文

【摘要】：为了研究种子油脂及脂肪酸生物合成机制,特别是α-亚麻酸的高水平积累,采用气相色谱法分析7个紫苏品种的成熟种子及晋苏1号种子不同发育时期脂肪酸组成、含量及其动态变化;实时荧光定量PCR检测TAG生物合成关键酶基因在紫苏不同组织及种子发育不同时期的表达特性,分析基因表达与脂肪酸及α-亚麻酸合成积累之间的关系。结果表明,紫苏种子中不饱和脂肪酸(18∶1,18∶2,18∶3)含量占总脂肪酸90%以上,种子发育过程中,总脂肪酸含量随着鲜重增加而不断增加,种子形态也经历由多水透明状转变为乳白色或深紫色最后成为褐色;随着种子不断发育,亚油酸含量逐渐降低,而α-亚麻酸含量不断增加,到种子成熟时达总脂肪酸质量的60%以上。晋苏1号作为高含α-亚麻酸的优质品种,α-亚麻酸含量达456.6mg/g,亚麻酸与亚油酸之比为9∶1。Real-time PCR分析结果显示Pf DGAT1和Pf PDAT在紫苏茎和根系中的表达量均很低,而在叶片和种子中高量表达,在种子发育不同时期,Pf DGAT1表达量呈先升高后降低的趋势,开花后30 d,表达量最高。Pf DGAT1基因超量表达之后紧接着α-亚麻酸和总油脂快速积累,表明Pf DGAT1在紫苏α-亚麻酸和总油脂积累过程中起关键作用。
[Abstract]:In order to study the biosynthesis mechanism of seed oil and fatty acid, especially the high level accumulation of 伪 -linolenic acid, the fatty acid composition of mature seeds of 7 perilla varieties and Jinsu-1 seed was analyzed by gas chromatography. The expression of key enzyme genes of TAG biosynthesis in different tissues of Perilla perilla and at different stages of seed development were detected by real-time fluorescence quantitative PCR. The relationship between gene expression and synthesis and accumulation of fatty acids and 伪 -linolenic acid was analyzed. The results showed that the content of unsaturated fatty acids in perilla seeds accounted for more than 90% of total fatty acids. The content of total fatty acids increased with the increase of fresh weight, and the morphology of seeds changed from water-transparent to milky white or dark purple to brown, and the content of linoleic acid decreased gradually with the development of seeds. And the content of 伪 -linolenic acid is increasing, The content of 伪 -linolenic acid was 456.6 mg / g, and the ratio of linolenic acid to linoleic acid was 9: 1. Real-time PCR analysis showed that the ratio of PF DGAT1 and PF PDAT in perilla stem was 9: 1. Real-time PCR analysis showed that the content of 伪-linolenic acid was 456.6 mg / g. The ratio of linolenic acid to linoleic acid was 9: 1. Real-time PCR analysis. And the amount of expression in the root system is very low, However, in leaves and seeds, the expression of PF DGAT1 increased first and then decreased at different stages of seed development. At 30 days after anthesis, the highest expression of .PF DGAT1 gene was overexpressed, followed by rapid accumulation of 伪 -linolenic acid and total oil, followed by rapid accumulation of 伪 -linolenic acid and total oil. These results indicate that PF DGAT1 plays a key role in the accumulation of 伪-linolenic acid and total oil in perilla.
【作者单位】：山西农业大学农学院;
【基金】：国家青年科学基金(31201266)
【分类号】：S567.219

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