蜚蠊肠道放线菌的卤化酶基因检测及初步分析

发布时间：2018-03-10 19:44

  本文选题：蜚蠊　切入点：放线菌　出处：《生物技术》2017年05期 　论文类型：期刊论文

【摘要】：[目的]对159株蜚蠊肠道内生放线菌的卤化酶等相关基因进行检测和初步分析。[方法]活化蜚蠊肠道内生放线菌,制备各菌株基因组DNA,根据依赖黄素腺嘌呤二核苷酸FADH2的卤化酶基因保守区设计简并引物,通过PCR扩增卤化酶基因片段,TA克隆后进行测序鉴定。在此基础上,对卤化酶阳性菌株进行聚酮合酶PKSⅠ和非核糖体多肽合成酶NRPS等2个次级代谢产物生物合成主要基因进行检测。[结果]159株蜚蠊肠道放线菌有84株含有卤化酶基因,占比52.83%;卤化酶基因阳性放线菌中71株含有PKSⅠ基因,70株含有NRPS,占比分别为84.52%、83.33%。[结论]蜚蠊肠道含有较丰富的卤化酶基因阳性放线菌,可作为未来分离卤化活性物质的微生物资源。
[Abstract]:[objective] to detect and analyze the halogenase and other related genes of 159 strains of cockroach intestinal actinomycetes. [methods] Activation of intestinal actinomycetes from cockroaches. The genomic DNAs of each strain were prepared. Degenerate primers were designed according to the conserved region of the halogenase gene dependent on the FADH2 of Flavin adenine dinucleotide. The halogenase gene fragment was cloned by PCR and sequenced. Two secondary metabolite biosynthesis genes, including polyketone synthase (PKS 鈪，

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