过度煎炸油脂对大鼠糖脂代谢及相关基因表达的影响

发布时间：2018-03-11 01:23

  本文选题：过度煎炸油　切入点：糖脂代谢　出处：《中国粮油学报》2017年04期 　论文类型：期刊论文

【摘要】：取24只生长状况相同的Wistar雄鼠随机分成3组,每天分别灌胃1.5 mL正常油脂、过度煎炸油脂,对照组灌胃等量的生理盐水。4周后眼眶取血,6周后腹部主动脉取血,用于各项生化指标的检测;取肝脏组织进行基因表达状况的检测。结果显示:干预4周后,与对照组相比,正常油脂组血脂含量均有所升高,过度煎炸油脂组基本与对照组持平;干预6周后,正常油脂组甘油三酯高于对照组,而过度煎炸油脂组甘油三脂、总胆固醇、高密度脂蛋白胆固醇水平均明显低于对照组(P0.01)。血糖水平在对照组、正常油脂组、过度煎炸油脂组呈逐渐增高趋势。qPCR结果表明:短期食用过度煎炸油脂即会导致大鼠肝脏组织ACC、ACOX1基因显著下调(P0.05或P0.01),这说明过度煎炸油脂中的毒性物质能通过影响脂肪酸正常的合成和代谢,造成机体脂肪酸合成代谢紊乱。同时Insig1基因明显下调(P0.01),说明过度煎炸油脂会对机体糖代谢产生一定影响。
[Abstract]:Twenty-four male Wistar rats with the same growth status were randomly divided into 3 groups. 1.5 mL of normal fat was fed into the stomach each day, and the control group was fed with the same amount of normal saline for 4 weeks, and the abdominal aorta was taken after 6 weeks of abdominal aorta blood collection after the control group was fed with the same amount of normal saline for 4 weeks. The results showed that after 4 weeks of intervention, compared with the control group, the serum lipids in the normal fat group were higher than those in the control group. After 6 weeks of intervention, triglycerides in the normal fat group were higher than those in the control group, while triglycerides and total cholesterol in the excessive frying fat group were higher than those in the control group. The level of high density lipoprotein cholesterol was significantly lower than that of control group (P 0.01). The results of qPCR showed that short-term overfrying oil could lead to the decrease of ACC-ACOX1 gene in liver tissue of rats by P0.01-P 0.05 or P0.01-P, which indicated that the toxic substances in overfried oil could be affected by the effect of overfrying oil. Normal synthesis and metabolism of fatty acids, At the same time, Insig1 gene obviously down-regulated P0.01G, which indicated that excessive frying and frying oil would have a certain effect on body glucose metabolism.
【作者单位】： 天津科技大学食品工程与生物技术学院;
【基金】：国家自然科学基金(31471701)
【分类号】：TS221
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本文编号：1595964