IgA肾病大鼠肾组织中T细胞免疫球蛋白与黏蛋白域基因1的表达和意义

发布时间：2018-03-11 08:03

本文选题：肾小球肾炎　切入点：Ig　出处：《中国现代医学杂志》2017年19期 　论文类型：期刊论文

【摘要】：目的观察Ig A肾病(Ig AN)大鼠肾组织中T细胞免疫球蛋白与黏蛋白域基因1(TIM-1)的表达,探讨TIM-1在Ig AN大鼠发病中的作用。方法 20只雄性SD大鼠随机分成Ig AN模型组和对照组,每组10只。在复制模型完成后处死大鼠,取肾脏,肾组织切片行PAS染色,免疫荧光观察大鼠肾脏病理变化,并行免疫组织化学法检测肾组织中TIM-1的表达,实时荧光定量聚合酶链反应(q RT-PCR)检测TIM-1 m RNA的表达。结果 Ig AN组TIM-1主要分布在肾小球和肾小管,表达量较对照组增加(P0.05)。Ig AN组肾组织中TIM-1 m RNA表达量均较对照组升高。TIM-1 m RNA相对表达量和肾脏病理Katafuchi评分呈正相关,且差异具有统计学意义(P0.05)。结论 TIM-1可能参与了Ig AN的发生发展。
[Abstract]:Objective to observe the expression of T cell immunoglobulin and mucin domain gene 1 (TIM-1) in the kidney of IgA nephropathy rats and to explore the role of TIM-1 in the pathogenesis of IgAN. Methods Twenty male SD rats were randomly divided into IGAN model group and control group. Ten rats in each group were killed after the completion of the model. PAS staining was performed on the kidneys and sections of the kidneys. The pathological changes of the kidneys were observed by immunofluorescence, and the expression of TIM-1 in the renal tissues was detected by immunohistochemical method. The expression of TIM-1 m RNA was detected by real-time fluorescence quantitative polymerase chain reaction Q RT-PCR.Results TIM-1 in IgAN group was mainly distributed in glomeruli and tubules. Compared with the control group, the expression of TIM-1 m RNA in renal tissue of P0.05 + IgAN group was significantly higher than that of control group. The relative expression of TIM-1 m RNA was positively correlated with the renal pathological Katafuchi score. The difference was statistically significant (P 0.05). Conclusion TIM-1 may be involved in the occurrence and development of IgAN.
【作者单位】：南昌大学第一附属医院肾内科;
【基金】：江西省自然科学基金(No:20142BAB205006) 江西省教育厅科学技术研究项目(No:14005)
【分类号】：R692.31

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