毛竹生长素反应因子基因的生物信息学分析及差异表达

发布时间：2018-03-12 17:35

本文选题：林木育种学　切入点：毛竹　出处：《浙江农林大学学报》2017年04期 　论文类型：期刊论文

【摘要】：生长素反应因子(ARF)基因家族在植物的生长发育中起至关重要的作用。关于毛竹Phyllostachys edulis ARF基因家族在花器官中生物信息学分析未见报道。以毛竹花器官为材料,采用生物信息学的方法,对毛竹中ARF基因进行筛选,并对其系统进化关系、保守基序及在花器官中的差异表达模式进行了初步的分析。结果表明:毛竹全基因组中含有44个ARF基因,分为Ⅰ,Ⅱ,Ⅲ类。与拟南芥Arabidopsis thaliana和水稻Oryza sativa分别比较,发现毛竹与水稻存在11个姐妹同源基因对。此外,Phe ARF13,Phe ARF14和Phe ARF35在花芽、雌蕊和幼胚中高量表达,同时Phe ARF2,Phe ARF30,Phe ARF14,Phe ARF13,Phe ARF35,Phe ARF7,Phe ARF37,Phe ARF38在雌蕊和幼胚中高量表达,推测这些基因可能在花发育和种子发育中发挥重要的作用。
[Abstract]:Auxin response factor (ARF) gene family plays a crucial role in the growth and development of plants. Phyllostachys edulis on bamboo ARF gene family in flower organ bioinformatics analysis has not been reported. In bamboo flower organ as materials, using the methods of bioinformatics, the bamboo ARF gene were screened, and the system evolution the relationship, analyzed the expression pattern of conserved motifs and differences in floral organs. The results showed that: Bamboo genome contains 44 ARF genes, divided into I, II and III respectively. Compared with Arabidopsis Arabidopsis thaliana and rice Oryza sativa, found that the bamboo and rice there are 11 sisters of homologous gene. In addition, Phe ARF13, Phe ARF14 and Phe ARF35 in flower bud, expression of pistil and embryo at the same time, Phe ARF2, Phe ARF30, Phe ARF14, Phe ARF13, Phe ARF35, Phe ARF7, Phe ARF37, Phe ARF38 in female The high expression of pistil and immature embryos suggests that these genes may play an important role in flower development and seed development.

【作者单位】：国际竹藤中心国家林业局竹藤科学与技术重点开放实验室;
【基金】：国家高技术研究发展计划(“863”计划)项目(2013AA102607-4) 国家自然科学基金资助项目(31570673)
【分类号】：S795.7

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