抗A亚群禽白血病受体基因在三个鸡群中分布的研究

发布时间：2018-03-14 13:08

本文选题：禽白血病病毒受体A基因　切入点：易感等位基因　出处：《黑龙江畜牧兽医》2017年05期 　论文类型：期刊论文

【摘要】：为了研究抗A亚群禽白血病病毒受体基因(Tva)易感与抗性等位基因在太行鸡、坝上长尾鸡和商品蛋鸡三个群体中的分布,试验采用直接测序法进行基因分型,并利用Popgene程序进行群体遗传分析。结果表明:在三个群体中共检测到4种等位基因(Tva~s、Tva~(r2)、Tva~(r5)和Tva~(r4)),等位基因Tva~s/Tva~(r2)在太行鸡中的频率为0.90/0.10,在坝上长尾鸡和商品蛋鸡中的频率为1.00/0.00;等位基因Tva~s/Tva~(r5)/Tva~(r4)在太行鸡中的频率为0.27/0.32/0.41,在坝上长尾鸡中的频率为0.44/0.38/0.18。太行鸡和坝上长尾鸡群体的多态位点均处于哈代温伯格平衡状态,在商品蛋鸡群中只检测到等位基因Tvas。说明太行鸡和坝上长尾鸡两个群体中存在不同频率的Tva抗性等位基因。
[Abstract]:In order to study the distribution of susceptible and resistant alleles of Avian Leukosis virus receptor Gene (TvaA) in three populations of Taihang Chicken, Bashang long-tailed hens and commercial layer hens, direct sequencing was used for genotyping. The results of population genetic analysis using Popgene program showed that four alleles, Tvasr2, Tvaanr5) and Tvaanus r4, were detected in three populations, and the frequency of the allele Tvasr / Tvao r2 was 0.90 / 0.10 in Taihang chickens and 0.90 / 0.10 in the long tail hens and commercial hens in the three populations, and the frequency of the alleles was 0.90 / 0.10 in the Taihang chickens, and the frequency of the alleles was 0.90 / 0.10 in the Taihang chickens. The frequency of the allele Tvaanus / Tvaanr5r5 / Tvar4 was 0.27 / 0.32 / 0.41 in Taihang chicken and 0.44 / 0.38 / 0.18in long tail chicken in Bashang, respectively. The polymorphic loci of Taihang chicken and Bashang long-tailed chicken population were in Hardy Weinberg equilibrium state, and the frequency was 0.44% 0.38% 0.18 in Taihang Chicken and Bashang long tail Chicken population, and the polymorphic loci of Taihang Chicken and Bashang long tail Chicken population were in Hardy Weinberg equilibrium. Only alleles Tvasa were detected in commercial laying hens, indicating that there were different frequencies of Tva resistance alleles in Taihang Chicken and Bashang long tail Chicken.
【作者单位】：河北农业大学动物科技学院;河北科技师范学院动物科技学院;
【基金】：河北省首批青年拔尖人才支持计划项目(2013-2015) 河北省现代农业产业技术体系蛋鸡产业创新团队项目河北农业大学中青年骨干教师境外研修项目
【分类号】：S858.31

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