蚯蚓Sox2基因的克隆及其在再生进程中的表达

发布时间：2018-03-14 18:23

本文选题：赤子爱胜蚓　切入点：Sox　出处：《动物学杂志》2016年01期 　论文类型：期刊论文

【摘要】：为了解Sox2基因在赤子爱胜蚓(Eisenia foetida)再生进程中的作用,本研究采用c DNA末端快速扩增(RACE)方法成功克隆并得到了完整的赤子爱胜蚓Sox2基因序列(Gen Bank登录号:KP637161),其c DNA序列全长2 354 bp,其中包括367 bp的5′端非翻译区,844 bp的3′端非翻译区和编码380个氨基酸残基的1 143 bp开放阅读框。通过实时荧光定量PCR检测了Sox2基因在发育成熟的赤子爱胜蚓不同体段(头部、环带和尾部)以及在尾部体段再生进程中的表达特征。结果显示:Sox2在不同体段中(头部、环带、尾部)的表达差异不显著。在尾部断肢后再生进程中,随着时间推移,Sox2的表达量明显上调,其中,截断后12 h,Sox2基因表达量达到峰值,是截断初期(0 h)的22倍。研究结果表明,Sox2基因可能与蚯蚓的再生进程有关。
[Abstract]:In order to understand the role of Sox2 gene in the regeneration process of Eisenia foetida, In this study, the rapid amplification of c DNA terminal was used to clone and obtain the complete Sox2 gene sequence of Aisheng wormworm, Genin Bank accession number: KP637161. The c DNA sequence was 2 354 BP in length, including 367bp 5'untranslated region 3' 844bp. The untranslated region and 1 143bp open reading frame encoding 380 amino acid residues were used to detect the different body segments of the Sox2 gene in the mature wormworm (head) by real-time fluorescence quantitative PCR. The results showed that there was no significant difference in the expression of w Sox2 in different body segments (head, ring, tail). With the passage of time, the expression of Sox2 was significantly up-regulated, and the peak expression of Sox2 gene reached its peak at 12 h after truncation, which was 22 times higher than that in the early period of truncation. The results showed that the Sox2 gene might be related to the regeneration process of earthworms.
【作者单位】：海南大学农学院;海南大学海口市环境毒理学重点实验室;中国农业大学生物学院农业生物技术国家重点实验室;海南大学环境与植物保护学院;
【基金】：海南省普通高等学校研究生创新科研课题(No.S201305)
【分类号】：Q786

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