18β-甘草次酸对HepG2肝癌细胞凋亡及c-jun基因表达的影响
发布时间：2018-03-15 00:32
本文选题：18β-甘草次酸 切入点：HepG2肝癌细胞 出处：《北方民族大学》2016年硕士论文 论文类型：学位论文
【摘要】：以往研究表明,18β-甘草次酸(18β-Glycyrrhetinic acid,18β-GA)有一定的防癌和抗癌作用,但对其分子机制的研究报道甚少。本课题组前期研究显示,18β-GA能够显著抑制HepG2肝癌细胞的增殖,并可调节一些癌症相关基因的表达。在此基础上,本论文研究了18β-GA对HepG2肝癌细胞凋亡及其对细胞凋亡相关基因c-jun表达的影响,初步探讨了18β-GA对HepG2肝癌细胞凋亡的影响及其分子机制。本论文主要研究结果如下:1、18β-GA对HepG2肝癌细胞的凋亡诱导作用。分别以60μg/mL的18β-GA处理HepG2肝癌细胞0 h、6 h、12 h、24 h、48 h(0 h组为对照组),之后采用流式细胞术,检测18β-GA对HepG2肝癌细胞的凋亡诱导作用。结果显示,与对照组相比,随着作用时间的延长,凋亡诱导率逐渐升高到10.09%、31.79%、42.2%和66.91%,且具有显著性差异(P㩳0.05)。该结果表明,18β-GA对HepG2肝癌细胞有时间依赖性的凋亡诱导作用。2、18β-GA对Hep G2肝癌细胞中c-jun基因表达的影响。分别以60μg/m L的18β-GA处理HepG2肝癌细胞0 h、6 h、12 h、24 h、48 h(0 h组为对照组)。采用western blotting和real time-PCR,分别检测了c-jun基因的mRNA和蛋白表达变化。结果显示,与对照组相比,18β-GA处理6 h,12 h,24 h,48 h后,cjun mRNA表达量分别上调了2.5倍(P㩳0.05)、2.7倍(P㩳0.05)、3.2倍(P㩳0.05)和6.2倍(P㩳0.05);c-jun蛋白表达量别上分调了3.8倍、6.2倍、8.4倍(P㩳0.05)和17.0倍(P㩳0.05)。该结果表明18β-GA处理能显著上调HepG2肝癌细胞中c-jun的基因表达,并且趋势与细胞凋亡趋势呈正相关,提示18β-GA对HepG2细胞凋亡的诱导作用可能与其上调c-jun基因的表达有关。
[Abstract]:Previous studies have shown that 18 尾 -Glycyrrhetinic acidinic 18 尾 -GA) has a certain anti-cancer and anticancer effect, but there are few reports on its molecular mechanism. Our previous studies have shown that Zeng18 尾 -GA can significantly inhibit the proliferation of HepG2 hepatoma cells. On the basis of this, the effect of 18 尾 -GA on apoptosis and c-jun expression of HepG2 hepatoma cells was studied. The effect of 18 尾 -GA on apoptosis of HepG2 hepatoma cells and its molecular mechanism were preliminarily studied. The main results of this study were as follows: 1: 1G 尾 -GA induced apoptosis in HepG2 hepatoma cells. HepG2 hepatoma cells were treated with 18 尾 -GA at 60 渭 g / mL for 0 h, 6 h, 12 h, 24 h and 48 h, respectively. The control group was treated with flow cytometry. The apoptosis-inducing effect of 18 尾 -GA on HepG2 hepatoma cells was detected. The results showed that the apoptosis-inducing rate increased gradually to 42.2% and 66.91% with the prolongation of treatment time, and there was significant difference between the two groups. The results showed that the apoptosis of HepG2 hepatoma cells was induced in a time-dependent manner. (2) 18 尾 -GA had an effect on the expression of c-jun gene in HepG2 hepatoma cells. HepG2 hepatoma cells were treated with 60 渭 g / mL 18 尾 -GA for 0 h, 6 h, 12 h, 24 h and 48 h 0 h, respectively. Western blotting and real time-PCR were used to detect the changes of mRNA and protein expression of c-jun gene. Compared with the control group, the expression of cjun mRNA was up-regulated by 2.5-fold, respectively, after treatment with 18 尾 -GA for 6 h, 12 h, 24 h and 48 h, respectively. P0. 05 and 2. 7 times P? P0. 05 and 3. 2 times P? 0. 05) and 6. 2 times P? The expression of c-jun protein was increased by 3.8 times, 6.2 times and 8.4 times, respectively. 0. 05) and 17. 0 times P? The results showed that 18 尾 -GA could significantly up-regulate the expression of c-jun gene in HepG2 hepatoma cells, and the trend was positively correlated with the trend of apoptosis, suggesting that 18 尾 -GA could induce apoptosis of HepG2 cells by up-regulating c-jun gene expression.
【学位授予单位】：北方民族大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R735.7
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本文编号：1613662
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