血清miR-17-92基因簇对视网膜母细胞瘤诊断价值的研究

发布时间：2018-03-15 23:36

本文选题：视网膜母细胞瘤　切入点：微小核糖核酸　出处：《中国当代儿科杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的分析血清mi R-17-92基因簇对视网膜母细胞瘤(RB)的诊断价值。方法采用实时定量PCR法检测20例RB患儿、20名健康对照者的血清mi R-17-92基因簇表达水平,分析其在不同分期RB患儿的表达差异及综合治疗前后的变化,并采用ROC曲线判断其对RB的诊断价值。结果 RB患儿血清中mi R-17-92基因簇成员mi R-17-3P、mi R-17-5P、mi R-18a和mi R-20a的相对表达量均高于健康对照组,以mi R-18a升高更为显著,差异均有统计学意义(P0.05);两组mi R-19a、mi R-19b-1和mi R-92a-1相对表达量的差异无统计学意义(P0.05)。mi R-17-5P、mi R-17-3P、mi R-18a和mi R-20a表达量在早中期和晚期RB患儿之间的差异均无统计学意义(P0.05),但综合治疗后表达量显著降低(P0.05)。mi R-17-3P、mi R-17-5P、mi R-18a和mi R-20a对RB诊断价值的曲线下面积(AUC)分别为0.770、0.755、0.828和0.665,以mi R-18a的AUC最大,并具有较好的灵敏度(90%)和特异度(65%)。结论 mi R-17-92基因簇的mi R-17-3P、mi R-17-5P、mi R-18a和mi R-20a在RB患儿血清中高表达,mi R-18a有可能作为RB诊断的新指标。
[Abstract]:Objective to analyze the diagnostic value of serum miR-17-92 gene cluster in retinoblastoma. Methods the expression of serum mi R-17-92 gene cluster in 20 children with RB and 20 healthy controls was detected by real-time quantitative PCR. The difference of RB expression in different stages and the changes before and after comprehensive treatment were analyzed. Results the relative expression of miR-17-92 gene cluster in serum of children with RB was higher than that of healthy controls, especially the increase of miR-18a. There was no significant difference between the two groups in the relative expression levels of mi R-19aanmi R-19b-1 and mi R-92a-1. There was no significant difference between the two groups in the relative expression of P0.05U. Mi R-17-5PnmMI-17-3Pangmi R-18a and miR-20a in the early, middle and late stages of RB, but there was no significant difference between the two groups in the early, middle and late stages of RB, but there was no significant difference between the two groups in the expression of R-92a-1, but there was no significant difference between the two groups in the combined therapy. The area under the curve of P 0.05, mi R-17-3, mi R-17-5, Pi, R-18a and mi R-20a for the diagnostic value of RB were 0.770 and 0.7550.6828 and 0.665, respectively, with the AUC of mi R-18a being the largest. Conclusion the high expression of mi R-17-92 gene cluster, mi R-17-3P, mi R-5P, mi R-20a, in the serum of RB children, may be a new marker for the diagnosis of RB, and has good sensitivity (90%) and specificity of 650.Conclusion the high expression of miR-17-92 gene cluster in the serum of RB patients may be a new marker for the diagnosis of RB.
【作者单位】：暨南大学第二临床学院暨深圳市人民医院儿科;
【基金】：深圳市卫生计生系统科研项目(编号:20151007)
【分类号】：R739.7

【相似文献】