Aurora家族基因在急性白血病细胞中的表达及其临床意义

发布时间：2018-03-16 01:14

本文选题：急性白血病　切入点：Aurora家族基因　出处：《中国实验血液学杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的:研究Aurora-A、B、C(AUR-A、B、C)基因mRNA在急性白血病中的表达情况及其与临床指标的相关性。方法:采用荧光定量PCR检测73例初诊急性白血病(AL)患者(初诊组)、20例化疗完全缓解急性白血病患者(缓解组)和骨髓单个核细胞中AUR-A、B、C mRNA表达水平,14例健康志愿者作为对照(健康组),并分析它们分别在不同分组中的表达性差异,与临床指标的相关性及AUR-A、B、C mRNA表达水平之间的相关性。结果:AURA、B、C mRNA表达在初诊组均高于健康组和缓解组(P0.01),健康组和缓解组3种基因mRNA表达水平差异均无统计学意义(P0.05),AUR-A、B、C 3种基因mRNA表达水平在急性淋巴细胞白血病(ALL)组均高于急性髓系白血病(AML)组(P0.01);AUR-A、B、C mRNA表达在高危组均高于低危组(P0.05),在高危组与中危组及中危组与低危组之间差异无统计学意义(P0.05);AUR-A、B、C表达在CD34、CD71、CD56阴性组和阳性组差异无统计学意义(P0.05),在不同性别、染色体、1疗程化疗是否缓解分组中差异无统计学意义(P0.05)。73例初诊AL患者中,AUR-A mRNA表达水平与初诊乳酸脱氢酶(LDH)水平和危险分层呈显著正相关(r=0.279,P=0.017;r=0.314,P=0.007);AUR-B与初诊LDH水平和危险分层呈显著正相关(r=0.277,P=0.018;r=0.349,P=0.002),AUR-A、B均与年龄、初诊白细胞(WBC)水平、初诊骨髓幼稚细胞比例和1疗程是否缓解无显著相关性;AUR-C表达水平与初诊白细胞水平、初诊LDH水平和危险分层显著正相关(r=0.263,P=0.025;r=0.348,P=0.003;r=0.376,P=0.001),与年龄显著负相关(r=-0.241,P=0.040),而与初诊骨髓中幼稚细胞比例和1疗程是否缓解无显著相关性;AUR-A和B(r=0.444,P=0.000)、AUR-B和C(r=0.763,P=0.000)、AUR-A和C(r=0.616,P=0.000)mRNA表达水平均呈显著正相关。结论:AUR-A、B、C mRNA在初诊急性白血病患者中高表达并且三者间表达水平显著正相关,其高表达与白血病类型、危险分层、初诊白细胞和乳酸脱氢酶水平等指标相关,均可作为预后指标和潜在治疗靶点
[Abstract]:Objective: to study the expression of Aurora-Aur-Amb BU C gene mRNA in acute leukemia and its correlation with clinical indexes. Methods: fluorescence quantitative PCR was used to detect 73 cases of newly diagnosed acute leukemia patients (newly diagnosed group, 20 cases with acute remission of chemotherapy). The expression of AUR-An BU C mRNA in leukemic patients (remission group) and bone marrow mononuclear cells (BMC) was analyzed in 14 healthy volunteers as control group (healthy group), and their differences in expression in different groups were analyzed. Results the expression of Aur-Amb C mRNA in the first diagnosis group was higher than that in the healthy group and the remission group (P 0.01). There was no significant difference in the expression of mRNA between the healthy group and the remission group (P 0.05) and the AUR-ABC expression level of AUR-ABC was not significantly different between the healthy group and the remission group, and the expression of AUR-ABC in the control group was significantly higher than that in the control group (P 0.05). The mRNA expression level of three genes in acute lymphoblastic leukemia (ALL) group was higher than that in acute myeloid leukemia (AMLL) group. The expression of mRNA in high risk group was higher than that in low risk group (P 0.05), but there was no significant difference between high risk group and moderate risk group and middle risk group and low risk group. There was no significant difference between CD34, CD71, CD56 negative group and positive group (P 0.05). There was no significant difference in the remission of chromosome 1 course chemotherapy. There was a significant positive correlation between the expression of AUR-A mRNA and the level of lactate dehydrogenase (LDHs) and risk stratification in 73 newly diagnosed AL patients. There was a significant positive correlation between AUR-A mRNA expression and newly diagnosed LDH levels and risk scores in patients with newly diagnosed AL. There was a significant positive correlation between the layer and age, and there was a significant positive correlation between the age and the age. There was no significant correlation between the expression of AUR-C and the level of newly diagnosed leukocytes, the proportion of immature bone marrow cells and the remission of one course of treatment. There was a significant positive correlation between LDH level and risk stratification at first diagnosis. There was a significant positive correlation between LDH level and risk stratification. There was a significant positive correlation between AUR-A and Brr0. 444P0. 000 AUR-B and Cr0. 763P0. 000AUR-A and Cr0. 616 P0. 000 mRNA expression levels. There was no significant positive correlation between the expression levels of AUR-A and Cr0. 616 P0. 000 P0. 000 mRNA levels in bone marrow and the remission of the first course of treatment. There was a significant positive correlation between AUR-A and Cr0. 763P0. 000A and Cr0. 616 P0. 000 mRNA levels. Conclusion the high expression of BMC mRNA in newly diagnosed acute leukemia patients is significant positive correlation among them. Its high expression was correlated with leukemia type, risk stratification, newly diagnosed leukocyte and lactate dehydrogenase level, and could be used as prognostic index and potential therapeutic target.
【作者单位】：郑州大学第一附属医院血液科;郑州大学医学科学院;郑州大学第一附属医院血液研究室;郑州大学第一附属医院郑州大学医学科学院;
【基金】：国家自然科学基金(813000418) 河南省科技厅攻关项目(132102310078)
【分类号】：R733.71

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