巴西蕉2个ERF转录因子基因的克隆及功能研究

发布时间：2018-03-16 04:07

本文选题：香蕉　切入点：AP/ERF　出处：《果树学报》2017年07期 　论文类型：期刊论文

【摘要】：【目的】筛选、克隆响应非生物胁迫的关键ERF转录因子基因,并研究其功能。【方法】综合运用转录组学、生物信息学、生物化学等技术手段,分析不同非生物逆境胁迫下AP2/ERF转录因子超家族基因的表达谱聚类变化,并从中筛选、克隆关键ERF转录因子,对其功能进行研究。【结果】不同非生物胁迫下,AP2/ERF超家族基因的表达模式不同,对低温胁迫的响应较其他胁迫敏感;筛选克隆的Ma ERF25和Ma ERF27基因是ERF家族基因,具备ERF家族基本特征,编码蛋白为核定位蛋白,C端具有转录激活活力,并参与了香蕉对非生物胁迫和激素的应答,在其中发挥着一定的作用。【结论】获得了2个巴西蕉ERF基因Ma ERF25和Ma ERF27,被定位在细胞核,具有转录激活活性,参与非生物逆境胁迫应答。
[Abstract]:[objective] to screen and clone the key ERF transcription factor genes in response to abiotic stress, and to study their functions. [methods] Transcriptome, bioinformatics, biochemistry and other techniques were used. The expression patterns of AP2/ERF transcription factor superfamily genes under different abiotic stress were analyzed and selected to clone the key ERF transcription factors. [results] the expression patterns of AP2 / ERF superfamily genes under different abiotic stress were different, and the response to low temperature stress was more sensitive than that of other stresses, and the cloned Ma ERF25 and Ma ERF27 genes were ERF genes. It has the basic characteristics of ERF family. The encoded protein is nuclear locator protein C terminal with transcriptional activation activity and participates in the response of banana to abiotic stress and hormone. [conclusion] two ERF genes Ma ERF25 and Ma ERF27 were obtained, located in the nucleus, with transcriptional activation activity and participating in abiotic stress response.
【作者单位】：中国热带农业科学院南亚热带作物研究所·海南省热带园艺产品采后生理与保鲜重点实验室;中国热带农业科学院热带生物技术研究所·农业部热带作物生物学与遗传资源利用重点实验室;
【基金】：现代农业产业技术体系(国家香蕉产业技术体系)建设专项(CARS-32) 973计划前期研究专项(2014CB160314) 香蕉、gg果、菠萝保鲜、贮运关键技术集成与产业化应用(ZDZX2013011)
【分类号】：S668.1

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