E-选择素基因A561C多态性与代谢综合征中医证型的相关性研究

发布时间：2018-03-17 00:02

本文选题：代谢综合征　切入点：E-选择素　出处：《药物生物技术》2015年06期 　论文类型：期刊论文

【摘要】：通过研究代谢综合征(Metabolic Syndrome,MS)患者不同中医证型与E-选择素基因A561C多态性的关系,为MS开展早期防治工作提供预测指标。方法:通过检测患者血清可溶性E-选择素水平,用聚合酶链反应-限制性片段长度多态性方法探查E-选择素基因A561C多态性在各组患者中的基因型和等位基因频率分布,对其与中医证型进行统计学相关性分析,通过分析得出代谢综合征患者各证型与E-选择素基因A561C多态性分布的规律的关系。(1)各临床参数间对比职业、年龄、BMI、高血压史、收缩压mm Hg、舒张压mm Hg、糖尿病史、空腹血糖(mmol/L)、肌酐(μmol/L)、可溶性E选择素的组间差异明显。(2)—般资料在不同证型组间的比较,只有性别的组间差异显著,男性明显多于女性。(3)实验组及对照组基因型分布均符合Hardy-Weinberg平衡(P0.05)。(4)E-选择素在痰瘀互结证中明显高于气滞湿阻证、气阴两虚证及脾肾气虚证这3组证型,而其他3组间的组间差异均不显著。E-选择素基因A561C多态性与代谢综合征中医证型密切相关,可以为中医辨证分型提供客观参考指标,并对此种疾病的早期防治具有指导意义。
[Abstract]:To study the relationship between Eselectin gene A561C polymorphism and different TCM syndromes in patients with metabolic syndromes (MSM), and to provide predictors for early prevention and treatment of MS. Methods: serum soluble Eselectin levels were detected in patients with metabolic syndrome. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the genotype and allelic frequency distribution of Eselectin gene A561C polymorphism in patients in each group, and the correlation between A561C polymorphism and TCM syndromes was analyzed statistically. The relationship between each syndrome type of metabolic syndrome and the distribution of E- selectin gene A561C polymorphism was analyzed. The clinical parameters were compared among occupation, age, hypertension, systolic blood pressure (mm), diastolic pressure (mm), diabetes mellitus (DM). Fasting blood glucose levels of mmol / L, creatinine (渭 mol / L, soluble E selectin) were significantly different among groups. The genotypes of male and control group were significantly higher than those of qi stagnation and dampness syndrome, Qi-yin deficiency syndrome and spleen and kidney qi deficiency syndrome. The polymorphism of E- selectin gene A561C is closely related to the TCM syndromes of metabolic syndrome, which can provide an objective reference index for the differentiation of TCM syndromes and have a guiding significance for the early prevention and treatment of this disease.
【作者单位】：南京市中医院;南京中医药大学;
【基金】：“江苏省中医药局科技项目”资助(No.LZ13111)
【分类号】：R259

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