茶树CsASR基因的克隆及其表达分析

发布时间：2018-03-17 08:29

本文选题：茶树　切入点：ASR基因　出处：《茶叶科学》2017年04期 　论文类型：期刊论文

【摘要】：逆境胁迫影响茶树生长发育及茶叶品质,ASR(abscisic acid,stress,ripening-induced)基因在植物抗逆响应中具有重要功能。本研究以龙井43品种茶树为材料,从中克隆了CsASR基因的全长cDNA序列、基因组序列及其启动子序列,分析了该基因的生物信息学特征及在组织间和不同胁迫处理下的表达模式。结果显示,CsASR的cDNA序列全长875 bp,含有546 bp的ORF序列,编码181个氨基酸,蛋白质分子量19.89 kD,理论等电点5.69;CsASR蛋白结构序列中74.5%的序列为无序结构,是一种无序蛋白;CsASR的C-端含有ABA/WDS功能结构域,主要定位于细胞质和细胞核中;茶树CsASR与海枣的ASR相似性最高,为87%,而在进化树中与枣的关系最近。CsASR基因含2个外显子,第1个外显子长363 bp,第2个外显子长183 bp,内含子较大为2 750 bp,内含子中含7种简单重复序列和2种DNA转座子序列。克隆获得起始密码子ATG上游2 554 bp的启动子区序列,该启动子上含有干旱、低温、高温以及ABA等相关的顺式作用元件。CsASR在根中的表达量最低;ABA抑制CsASR的表达,而干旱、NaCl和低温胁迫能够显著上调CsASR的表达。表明CsASR基因可能与茶树抗逆密切相关。
[Abstract]:The effects of stress stress on the growth and development of tea plants and the quality of tea were studied. The full-length cDNA sequence of CsASR gene was cloned from the stress response of tea plants. The genome sequence and its promoter sequence were analyzed. The bioinformatics characteristics of the gene and its expression patterns under different stress treatments were analyzed. The results showed that the cDNA sequence of CsASR was 875 BP in length and contained a ORF sequence of 546 BP, encoding 181 amino acids. The molecular weight of the protein was 19.89 kD, and the sequence of 74.5% in the theoretical isoelectric point of 5.69 ABA/WDS protein was disordered, and the C-terminal of the disordered protein contained ABA/WDS functional domain, which was mainly located in the cytoplasm and nucleus. The ASR similarity of tea tree CsASR and Chinese jujube was the highest (87%), while in the evolutionary tree, there were two exons in the CsASR gene. The first exon length was 363 BP, the second exon length was 183 BP, the intron was larger than 2 750 BP, and the intron contained 7 simple repeats and 2 DNA transposon sequences. The promoter sequence upstream of the start codon ATG was 2 554bp. The promoter contains drought, low temperature, high temperature and ABA related cis-acting elements. CsASR has the lowest expression in root and inhibits the expression of CsASR. However, drought stress and low temperature stress could significantly up-regulate the expression of CsASR, suggesting that CsASR gene may be closely related to the stress resistance of tea plants.
【作者单位】：福建农林大学园艺学院/茶学福建省高校重点实验室/中国乌龙茶协同创新中心;中国农业科学院茶叶研究所/国家茶树改良中心/农业部茶树生物学与资源利用重点实验室;
【基金】：国家自然科学基金(31600555) 福建省自然科学基金项目(2017J01616) 国家茶叶产业技术体系(CARS-23)
【分类号】：Q943.2;S571.1

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