sco1135基因对天蓝色链霉菌M145孢子形成及次级代谢产物合成的调控研究

发布时间：2018-03-17 12:28

本文选题：天蓝色链霉菌M　切入点：sco　出处：《生物技术通报》2017年01期 　论文类型：期刊论文

【摘要】：旨在研究sco1135基因缺失突变对天蓝色链霉菌M145菌株形态及次级代谢的影响。通过PCR-targeting方法获得重组质粒p SJ1135,通过接合转移将其导入天蓝色链霉菌M145,获得sco1135基因缺失突变菌株△sco1135,并以p MS82为载体构建回补菌株△sco1135com,同时以p MS82为空载对照;随后对野生型菌株、突变菌株和回补菌株进行表型分析和抗生素定量观察。结果显示,表型分析及抗生素定量测定发现,在YBP培养基上△sco1135产孢明显延迟于野生型M145,放线紫红素(ACT)产量明显增加,突变株培养基中ACT产量是野生菌株培养基中的2-3倍;转录分析结果表明,48 h时突变株部分与产孢相关基因的转录水平较野生型降低了50%-75%,72 h时突变株部分与产ACT相关基因的转录水平较野生型提高13-20倍。sco1135基因参与调控M145的孢子形成及次级代谢产物ACT的产生。
[Abstract]:The aim of this study was to study the effect of sco1135 gene deletion mutation on morphology and secondary metabolism of Streptomyces cereus strain M145. Recombinant plasmid pSJ1135 was obtained by PCR-targeting method, and then introduced into Streptomyces cereus M145 by conjugation transfer, and sco1135 gene deletion was obtained. The mutant strain sco1135 was constructed by using p MS82 as vector and p MS82 as no-load control. Then phenotypic analysis, phenotypic analysis and antibiotic quantitative observation of wild-type, mutant and compensatory strains were carried out, the results showed that phenotypic analysis and antibiotic quantitative analysis showed that, The sporulation of sco1135 on YBP medium was significantly delayed than that of wild type M145, and the yield of actinomycin (actinomycin) was obviously increased, and the yield of ACT in mutant medium was 2-3 times higher than that in wild strain medium. The results of transcriptional analysis showed that the transcriptional level of some of the genes associated with spore production of mutant strain at 48 h was lower than that of wild type, and the transcription level of part of mutant and ACT related gene was 13-20 times higher than that of wild type at 72h after -75h. Spore formation of M145 and production of secondary metabolite ACT.
【作者单位】：济南大学山东省医学科学院医学与生命科学学院;山东省医药生物技术研究中心;山东大学微生物技术国家重点实验室;

【相似文献】