人类免疫缺陷病毒合并结核杆菌感染者外周血差异表达基因的生物信息学分析

发布时间：2018-03-17 14:24

本文选题：人类免疫缺陷病毒　切入点：结核杆菌　出处：《中国医学科学院学报》2017年03期 　论文类型：期刊论文

【摘要】：目的分析人类免疫缺陷病毒(HIV)/结核杆菌(TB)感染者外周血基因的生物学网络调控机制及关键蛋白,为HIV/TB感染者的早期诊断和临床治疗提供新依据。方法从基因芯片公共数据库GEO中下载了22例南非HIV感染者和22例HIV/TB感染者的外周血基因芯片数据,将数据导入分析软件QOE、STRING、PANTHER、GenClip等,分析两组患者的基因表达谱、蛋白相互作用网络、分子生物学过程、基因功能等,寻找区别两类患者的关键节点基因。结果两组患者15 529个基因的表达谱相似,44名受试者的基因表达谱具有高度关联性;251个(1.62%)差异表达基因对诊断HIV/TB感染具有良好的诊断能力;RPLP1是蛋白-蛋白相互作用图谱子网络的核心节点;差异表达基因与正向调节外界刺激、细胞内信号通路的转导、中性粒细胞的迁移、人体免疫等有关,主要参与自由基相关凋亡、炎性反应等通路的活化。结论共发现251个差异表达的基因可将单纯HIV感染和HIV/TB感染进行区别,其中差异表达的前40个基因的蛋白-蛋白相互作用网络中所包含的RPLP1基因可能与HIV/TB感染密切关联,主要涉及正向调节外界刺激、细胞内信号通路的转导、中性粒细胞的迁移、人体免疫等生物学功能,为HIV/TB感染的诊断和治疗提供了一定的参考依据。
[Abstract]:Objective to analyze the biological network regulation mechanism and key protein of peripheral blood gene in human immunodeficiency virus (HIV) infected patients with HIV / Mycobacterium tuberculosis (TB) infection. Methods the peripheral blood gene chip data of 22 South African HIV infected patients and 22 HIV/TB infected persons were downloaded from GEO, a common database of gene chips, and the data were imported into the software QOERINGPANTHERN GenClip. Analysis of gene expression profiles, protein interaction networks, molecular biological processes, gene functions, etc. Results the expression profiles of 15 529 genes in the two groups were similar to those of 44 subjects. The gene expression profiles of 44 subjects had a high correlation.) the differentially expressed genes had a good value in the diagnosis of HIV/TB infection. RPLP1 is the core node of protein-protein interaction map network. Differentially expressed genes are related to positive regulation of external stimuli, transduction of intracellular signaling pathways, neutrophil migration, human immunity and so on, and are mainly involved in free radical related apoptosis. Conclusion 251 differentially expressed genes can distinguish between HIV infection and HIV/TB infection. The RPLP1 gene contained in the protein-protein interaction network of the first 40 differentially expressed genes may be closely related to HIV/TB infection, mainly involved in positive regulation of external stimulation, transduction of intracellular signal pathway, and neutrophil migration. Human immunity and other biological functions provide a certain reference for the diagnosis and treatment of HIV/TB infection.
【作者单位】：陕西中医药大学公共卫生学院流行病与卫生统计学教研室;陕西中医药大学第一临床医学院骨科;
【基金】：咸阳市科技局重点项目(2013K12-01) 陕西中医药大学青年基金项目(2015QN05)~~
【分类号】：R512.91

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