甘蓝型油菜DOF蛋白基因的克隆与生物信息学分析

发布时间：2018-03-18 01:03

  本文选题：甘蓝型油菜　切入点：Dof蛋白基因　出处：《北方园艺》2017年14期 　论文类型：期刊论文

【摘要】：Dof转录因子是植物特有的一类转录因子,含有一个独特的单锌指结构域,在植物的生长发育中起重要的调控作用。以甘蓝型油菜为试材,采用电子克隆和RT-PCR技术,以期克隆一个具有Zf-Dof结构域的蛋白基因cDNA序列。结果表明:该基因含有1284bp的开放阅读框,编码427个氨基酸,蛋白分子量为46.9kDa,等电点为8.86,为亲水性蛋白。经序列比对分析表明其与拟南芥CDF3基因有较高同源性,可能具有相似的功能。
[Abstract]:Dof transcription factors are plant specific transcription factors, which contain a unique single zinc finger domain and play an important role in plant growth and development. Using Brassica napus as a test material, electronic cloning and RT-PCR techniques were used. In order to clone the cDNA sequence of a protein gene with Zf-Dof domain, the results showed that the gene contained 1284bp open reading frame, encoding 427amino acids. The protein has a molecular weight of 46.9 kDa and an isoelectric point of 8.86, which is a hydrophilic protein. Sequence alignment analysis shows that the protein has high homology with Arabidopsis CDF3 gene and may have similar function.
【作者单位】： 遵义师范学院生物与农业科技学院;
【基金】：国家自然科学基金资助项目(31501337) 贵州省科学技术厅与遵义市科学技术局及遵义师范学院联合科技基金资助项目(黔科合LH字[2016]7014号) 遵义师范学院博士基金资助项目(遵师BS[2014]25号)
【分类号】：S565.4
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本文编号：1627280