反义4CL基因调节烟草木质素生物合成

发布时间：2018-03-18 09:18

本文选题：木质素　切入点：烟草　出处：《分子植物育种》2017年11期 　论文类型：期刊论文

【摘要】：采用根癌农杆菌介导法的叶盘转化法,将紫穗槐反义4CL基因片段导入烟草中,获得T0代阳性转化植株。T0代转基因植株自花授粉,收获种子,种植后获得T1代植株。PCR、RT-PCR(T1代)检测、目的片段测序的结果表明,反义4CL基因已经稳定遗传到T1代中。T1代烟草阳性植株和阴性植株的比例为77:23,接近于3:1。两株T1代烟草转化植株q RT-PCR及4CL酶含量检测表明,叶片4CL1基因表达量较野生植株降低了19.51%;茎4CL酶含量降低了10.50%,说明该反义基因能够干扰烟草4CL基因的表达。T1代烟草茎木质素含量平均下降了11.87%,根木质素含量降低了14.23%。种植60 d的转基因植株与野生型植株生长一致,在株高、株重等方面差异不显著(p0.05),说明反义4CL基因的转入在降低了木质素含量的同时并没有影响烟草的正常生长。
[Abstract]:Using Agrobacterium tumefaciens-mediated leaf disk transformation, the antisense 4CL gene fragment of Amorpha fruticosa was introduced into tobacco. The transgenic plants of generation T0 and generation T0 were self-pollinated and harvested seeds. After planting, T1 generation plants were obtained. Objective the results of fragment sequencing showed that the proportion of antisense 4CL gene to the positive and negative tobacco plants in T1 generation was 77: 23, which was close to 3: 1.The results of Q RT-PCR and 4CL enzyme contents of two T1 generation tobacco transformation plants showed that the antisense 4CL gene was stable in T1 generation. Leaf 4CL1 gene expression decreased 19.51% compared with wild plants, stem 4CL enzyme content decreased 10.50%, indicating that the antisense gene could interfere with tobacco 4CL gene expression. T1 generation tobacco stem lignin content decreased by 11.87%, root lignin content decreased. 14.23. the transgenic plants planted for 60 days grew the same as the wild-type plants. There was no significant difference in plant height and plant weight, indicating that the transfer of antisense 4CL gene decreased lignin content and did not affect the normal growth of tobacco.
【作者单位】：辽宁大学生命科学院;
【基金】：辽宁省自然科学基金(2012020283)资助
【分类号】：S572

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