烟草镉转运基因突变体的鉴定和功能分析

发布时间：2018-03-18 18:39

本文选题：烟草　切入点：TILLING技术　出处：《烟草科技》2017年09期 　论文类型：期刊论文

【摘要】：为获得对镉吸收能力下降的烟草材料,通过TILLING技术筛选出镉转运基因NtHMA2的突变体并测定了该突变体中镉及其他重金属的含量。结果表明,在镉胁迫下,与对照相比突变体烟叶中的镉含量降低36.84%;锌、铬、镍、砷、铁含量分别降低27.92%、32.48%、30.18%、11.33%、30.51%。突变体株高比对照显著降低,其他农艺性状无显著差异。获得的对镉吸收能力下降的突变体材料hma2-23可用于烟草抗镉胁迫品种的培育。
[Abstract]:In order to obtain tobacco materials with decreased cadmium absorption ability, the mutant of cadmium transporter gene NtHMA2 was screened by TILLING and the contents of cadmium and other heavy metals in the mutant were determined. The results showed that, under cadmium stress, cadmium and other heavy metals in the mutant were determined. Compared with the control, the content of cadmium in tobacco leaves decreased by 36.844.The contents of zinc, chromium, nickel, arsenic and iron decreased 27.92% and 32.48%, respectively. The plant height of the mutant was significantly lower than that of the control. There was no significant difference in other agronomic characters. Hma2-23, a mutant with decreased cadmium absorption ability, could be used to cultivate varieties resistant to cadmium stress in tobacco.
【作者单位】：云南省烟草农业科学研究院烟草行业烟草生物技术育种重点实验室国家烟草基因工程研究中心;
【基金】：中国烟草总公司科技重大专项“利用镉转运基因创制云烟87低镉品系”[110201601031(JY-05)] 中国烟草总公司云南省公司科技计划项目“利用镉转运基因创制云烟87低镉品系”(2017YN03)
【分类号】：S572;X173

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