维氏气单胞菌菌蜕疫苗免疫后鲤免疫应答与hepcidin基因表达特征

发布时间：2018-03-18 19:42

本文选题：维氏气单胞菌菌蜕　切入点：鲤(Cyprinus　出处：《微生物学通报》2017年06期 　论文类型：期刊论文

【摘要】：【目的】为探讨接种维氏气单胞菌菌蜕疫苗后鲤的应答反应特征及与甲醛灭活苗的效果比较,在实验条件下研究了接种疫苗后鲤(Cyprinus carpio)的特异性、非特异性免疫指标及hepcidin基因表达量的变化。【方法】在开始实验的第1、15和29天使用菌蜕疫苗(CLGs)和甲醛灭活疫苗(FKC)对鲤进行注射3次免疫,并设一个注射磷酸缓冲液(PBS)的对照组,每个组设3个重复。从第一次免疫后每隔7 d从每个重复中随机取三尾鱼进行取样,对血清抗体滴度、相对免疫保护率(RPS)、溶菌酶(LZM)、呼吸暴发、补体C3、髓过氧化物酶(MPO)、丙二醛(MDA)及hepcidin基因表达量进行测定。【结果】二次免疫后,CLGs组抗体凝集效价达2~6-2~8,显著高于FKC和PBS组(P0.05),且其呼吸暴发、LZM、MPO含量和hepcidin基因表达量显著高于PBS组(P0.05),MDA含量显著低于PBS组(P0.05);CLGs组C3含量在35和42 d显著低于PBS组(P0.05);FKC组MPO含量和hepcidin基因表达量显著低于菌蜕组(P0.05)。CLGs组的RPS为57.70%,FKC组RPS为30.77%。【结论】接种一定强度维氏气单胞菌菌蜕疫苗,能够提高鲤血清抗体效价和呼吸暴发、LZM、MPO等非特异性免疫指标及hepcidin基因表达,而且整体免疫效果好于甲醛灭活苗。
[Abstract]:[objective] to investigate the response characteristics of Cyprinus carpio after inoculation of Aeromonas viride slough vaccine and to compare its effect with that of formaldehyde inactivated vaccine, the specificity of Cyprinus carpio (Cyprinus Carpio) after inoculation was studied under experimental conditions. The changes of nonspecific immune indexes and the expression of hepcidin gene. [methods] Cyprinus Carpio was immunized three times with molt vaccine and formaldehyde inactivated vaccine on the 15th and 29th days of the experiment, and a control group was injected with phosphate buffer. Each group was divided into 3 replicas. Three fish were randomly sampled from each repeat every 7 days after the first immunization. The titer of serum antibody, relative immune protection rate (RPSN), lysozyme (LZM) and respiratory outbreak were measured. Complement C3, myeloperoxidase (MPO), malondialdehyde (malondialdehyde) and hepcidin gene expression were measured. [results] the antibody agglutination titers of hepcidin group after secondary immunization were significantly higher than that of FKC and PBS groups (P 0.05), and the content of MPO and hepcidin gene expression in the respiratory outbreak group were significantly higher than those in FKC and PBS groups. The content of MPO and hepcidin gene in PBS group was significantly lower than that in PBS group at 35 and 42 days. It was significantly lower than that in PBS group P0.05 FKC group and RPS 57.70 FKC group. [conclusion] there was a certain inoculation strength in Vickers group. [conclusion]. Aeromonas aeruginosa slough vaccine, It can improve the serum antibody titer of common carp and the expression of hepcidin gene and non-specific immune index such as LZM MPO, and the overall immune effect is better than that of formaldehyde inactivated vaccine.
【作者单位】：上海海洋大学国家水生动物病原库;北京市水产科学研究所;天津科技大学;
【基金】：北京市自然科学基金项目(No.6152006) 北京市观赏鱼产业创新团队项目(No.BAIC03-2016)~~
【分类号】：S948

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