四纹豆象及其近缘种基于COI基因的分子检测技术研究

发布时间：2018-03-19 01:35

本文选题：四纹豆象　切入点：分子检测　出处：《植物检疫》2017年03期 　论文类型：期刊论文

【摘要】：四纹豆象是口岸检疫中经常截获的种类,本文以四纹豆象及其近缘种为研究对象,测定分析了COI基因516 bp碱基序列。序列分析结果表明:保守位点为353个,变异位点为163个,简约信息位点为134个,自裔位点为29个。基于Kimura 2-parameter模型分析遗传距离,结果显示:种内遗传距离介于0.001~0.013之间,平均遗传距离为0.008,种间遗传距离介于0.114~0.193,平均遗传距离为0.161。采用邻接法构建的COI基因序列系统发育树显示,同一物种聚为同一小支,且分支自展值均为100%。结果表明应用COI基因片段对四纹豆象及其近缘种进行分子鉴定具有可行性。
[Abstract]:The COI gene 516bp base sequence was determined and analyzed in this paper. The results showed that there were 353 conserved sites and 163 mutation sites. The results of genetic distance analysis based on Kimura 2-parameter model showed that the genetic distance within species was between 0.001 and 0.013. The average genetic distance was 0.008, the interspecific genetic distance was 0.1140.1993, and the average genetic distance was 0.161.The phylogenetic tree of COI gene sequence constructed by the method of contiguity showed that the same species was clustered into the same small branch. The results showed that it was feasible to use the COI gene fragment to identify the four striped legume and its relative species.
【作者单位】：合肥工业大学食品科学与工程学院;安徽出入境检验检疫局;
【基金】：安徽省自然科学基金项目(1408085MC57) 中央高校基本科研业务费专项资金资助(JZ2015HGQC0210)
【分类号】：S41-30

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