硅对辣椒CaLEA5基因低温胁迫下表达分析

发布时间：2018-03-19 18:15

本文选题：硅酸钾　切入点：辣椒　出处：《基因组学与应用生物学》2016年12期 　论文类型：期刊论文

【摘要】：通常在逆境胁迫下,植物细胞会诱导产生一系列的逆境蛋白来保护细胞免受伤害,其中胚胎发育晚期丰富(LEA)蛋白是目前研究最为普遍的一种。为了研究辣椒LEA蛋白的耐寒性,以豫椒101为材料,根据已经报道的LEA基因序列设计引物,通过PCR技术从辣椒基因组中获得LEA基因,命名为CaLEA5。经生物信息学分析,该基因具有一个完整的ORF为297 bp,编码98个氨基酸。辣椒CaLEA5蛋白质含有LEA基因家族保守的结构域。对其亚细胞定位、跨膜结构进行分析,预测其定位在叶绿体,存在跨膜结构。荧光定量PCR检测结果表明,低温胁迫可诱导CaLEA5基因的表达,且其表达量在迅速达到峰值后又降低,说明CaLEA5是一个快速响应基因,推测其在辣椒抗逆机制中起着重要的作用。在低温胁迫的条件下,硅处理可延缓CaLEA5基因的上调表达。
[Abstract]:Usually under stress, plant cells induce a series of stress proteins to protect the cells from injury, among which the late embryonic development rich Lea protein is one of the most widely studied in order to study the cold tolerance of chili pepper LEA protein. Using Yujiao 101 as the material, primers were designed according to the reported LEA gene sequence, and the LEA gene was obtained from capsicum genome by PCR technique, named CaLEA5. the results were analyzed by bioinformatics. The gene has a complete ORF of 297bpand encodes 98 amino acids. The CaLEA5 protein of capsicum peppers contains the conserved domain of LEA gene family. Its subcellular localization and transmembrane structure are analyzed, and its localization in chloroplasts is predicted. The results of fluorescence quantitative PCR showed that the expression of CaLEA5 gene could be induced by low temperature stress, and its expression level decreased after reaching the peak rapidly, which indicated that CaLEA5 was a rapid response gene. It is speculated that it plays an important role in the stress resistance of capsicum. Under the condition of low temperature stress, silicon treatment can delay the up-regulation of CaLEA5 gene expression.
【作者单位】：河南省农业科学院园艺研究所;驻马店市农业科学院;
【基金】：国家大宗蔬菜产业技术体系豫北综合试验站项目(CARS-25-G-27) 农业部黄淮地区(河南)蔬菜科学观测实验站(10205020) 河南省重大科技专项(151100110400)共同资助
【分类号】：S641.3;Q943.2

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