益气养阴补肾方联合激素对自发狼疮转基因小鼠增效减毒作用的研究

发布时间：2018-03-19 21:43

本文选题：益气养阴补肾法　切入点：黄芪生脉二至饮　出处：《时珍国医国药》2017年05期 　论文类型：期刊论文

【摘要】：目的研究益气养阴补肾方-黄芪生脉二至饮配合激素对B6.MRL-Faslpr/NJU自发狼疮样转基因小鼠增效减毒作用,为构建益气养阴补肾方联合激素治疗系统性红斑狼疮的诊疗方案奠定理论基础。方法构建B6.MRL-Faslpr/NJU狼疮样转基因小鼠模型,按照蛋白尿水平平均分为模型组、激素组、黄芪生脉二至饮给药组和黄芪生脉二至饮联合激素给药组。给药10周后,酶联免疫吸附法(ELISA)法检测狼疮样转基因小鼠血清抗ds-DNA抗体水平,MTT法测定各组豆刀蛋白(Con A)诱导小鼠T淋巴细胞增殖功能;卡马斯亮蓝法检测尿蛋白含量变化;石蜡切片HE染色和免疫荧光法分别测定肾组织中病理损伤和免疫复合物沉积及分布情况;生化仪检测肾功能和计算脾指数。结果给药10周后,与病理模型组相比,黄芪生脉二至饮联合激素显著降低尿蛋白水平,减少肾组织免疫复合物沉积及病理损伤;与单用激素比较,黄芪生脉二至饮联合激素增加脾指数,显著改善激素诱导的免疫抑制状态。结论益气养阴补肾方联合激素具有显著的协同作用,既能改善实验室指标,又能显著改善激素诱导免疫抑制的副作用,因此值得SLE患者长期使用和推广。
[Abstract]:Objective to study the synergistic and attenuated effect of Huangqi Shengmai Erzhi decoction combined with hormone on B6.MRL-Faslpr-NJU spontaneous lupus transgenic mice. Methods B6.MRL-Faslpr-NJU transgenic mice model was constructed and divided into model group and hormone group according to the level of proteinuria. Huangqi Shengmai Erzhi decoction group and Huangqi Shengmai Erzhi decoction combined hormone administration group. 10 weeks after administration, The levels of serum anti-ds-DNA antibody in lupus-like transgenic mice were detected by enzyme-linked immunosorbent assay (Elisa) and the proliferative function of T lymphocytes induced by Con A was determined by MTT assay, and the changes of urine protein content were detected by Camassie brilliant blue method. Paraffin sections were stained with HE and immunofluorescence were used to determine the pathological injury and the deposition and distribution of immune complex in renal tissue, the renal function and spleen index were measured by biochemical instrument. Results after 10 weeks of administration, the pathological model group was compared with the pathological model group. Astragali Shengmai Erzhi decoction combined with hormone significantly decreased urinary protein level, reduced renal tissue immune complex deposition and pathological damage, compared with hormone alone, Astragalus Shengmai Erzhi decoction combined with hormone increased spleen index. Conclusion the combination of Yiqi Yangyin Bushen prescription and hormone can not only improve the laboratory indexes, but also improve the side effects of hormone induced immunosuppression. Therefore, it is worth using and popularizing SLE patients for a long time.
【作者单位】：云南中医学院;云南中医学院第一附属医院;云南省中医中药研究院;
【基金】：国家自然科学基金(No.81460624) 云南省教育厅科研基金重点项目(No.2013Z005)
【分类号】：R285.5

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