高温堆肥对猪粪中四环素类抗生素及抗性基因的影响

发布时间：2018-03-20 01:28

本文选题：四环素　切入点：四环素类抗性基因　出处：《环境科学学报》2017年04期 　论文类型：期刊论文

【摘要】：为了研究高温堆肥对猪粪中抗生素及抗生素抗性基因的影响,对不同堆肥处理中的四环素类抗生素(四环素、土霉素、金霉素)及其抗性基因(tetA、tetC、tetG、tetM、tetQ、tetW)的动态变化进行了定量研究.结果表明:低剂量组(10 mg·kg~(-1))中四环素、土霉素、金霉素的去除率分别为91%、94%、92%,高剂量组(50 mg·kg-1)中四环素、土霉素、金霉素的去除率分别为60%、62%、71%.在堆肥过程中,抗生素处理组目标基因相对丰度高于对照组,而低剂量组目标基因相对丰度(tetW除外)又普遍高于高剂量组.经堆肥处理后,tetA的相对丰度增加,tetC、tetG、tetM、tetQ、tetW的相对丰度均下降.高温堆肥可以有效去除猪粪中一定浓度的四环素类抗生素,并对四环素类抗性基因也有一定的削减作用.
[Abstract]:In order to study the effect of high temperature composting on antibiotics and antibiotic resistance genes in pig manure, tetracycline antibiotics (tetracycline, oxytetracycline) in different composting treatments were studied. The dynamic changes of chlortetracycline and its resistant gene tetetGntetGntetQtetW) were quantitatively studied. The results showed that the removal rates of tetracycline, oxytetracycline and chlortetracycline were 919492 in low dose group and 50 mg 路kg-1 in high dose group respectively, the results showed that the removal rates of tetracycline, oxytetracycline and oxytetracycline in high dose group were 50 mg 路kg ~ (-1), 10 mg 路kg ~ (-1) and 50 mg 路kg ~ (-1), respectively. The removal rates of chlortetracycline were 60% and 62% respectively. During composting, the relative abundance of target gene in antibiotic treatment group was higher than that in control group. However, the relative abundance of target gene in low dose group was higher than that in high dose group. After composting, the relative abundance of tete A increased, and the relative abundance of tete C tet Glutet Mtet QtetW decreased. High temperature compost could effectively remove certain concentrations of tetracycline antibiotics from pig manure. In addition, tetracycline resistance genes were also reduced to some extent.
【作者单位】：吉林农业大学动物科技学院;
【基金】：国家现代农业产业技术体系建设专项(No.CARS-38) 2013年公益性行业(农业)科研专项(No.201303091)~~
【分类号】：S141.4;X713

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