田鼠巴贝虫棒状体相关蛋白基因的筛选及生物信息学分析

发布时间：2018-03-20 05:24

本文选题：田鼠巴贝虫　切入点：棒状体相关蛋白　出处：《中国病原生物学杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的通过对专业数据库的搜索,筛选出田鼠巴贝虫棒状体相关蛋白的基因,并进行生物信息学分析预测。方法搜索NCBI、EMBL-EBI、Uniprot及PiroplasmaDB数据库,筛选田鼠巴贝虫棒状体蛋白基因。通过生物信息学方法,利用多种数据库及生物信息学分析软件分析其对应氨基酸序列的疏水区、跨膜区、信号肽及结构域,预测该蛋白二级结构、三级结构及其抗原表位;利用Clustal-X软件对其氨基酸序列进行同源性比对;利用PAUP软件构建系统发育树,对氨基酸序列进行多重序列比对(mutiple sequence alignment)。结果在EMBL-EBI和PiroplasmaDB中各筛选出1条田鼠巴贝虫棒状体蛋白基因,登录号分别为XP_012649548和BBM_III04695。通过软件APE进行序列比对,两个基因序列完全一致,证明为同一条基因序列。该基因共含有4 443bp,可编码1 480个氨基酸。编码蛋白分子质量为165.307ku,为分泌蛋白,含一个信号肽,4个跨膜区,并含有较多抗原表位。结论采用生物信息学方法预测田鼠巴贝虫棒状体相关蛋白含有较多的抗原表位,可作为疫苗和诊断抗原的候选分子。
[Abstract]:Objective to screen out the genes of coryloid related proteins of Barb 茅 worm in voles by searching the specialized database and to carry out bioinformatics analysis and prediction. Methods to search the databases of EMBL-EBIN Uniprot and PiroplasmaDB. To screen the coryloid protein gene of Barb's bug in voles, the hydrophobic region, transmembrane region, signal peptide and domain of amino acid sequence were analyzed by using various databases and bioinformatics analysis software. The secondary structure, tertiary structure and antigenic epitopes of the protein were predicted, its amino acid sequence was compared by Clustal-X software, and phylogenetic tree was constructed by PAUP software. Multiple sequence alignment of amino acid sequences was carried out. Results one coryloid protein gene of Barb 茅 a vole was screened from EMBL-EBI and PiroplasmaDB, respectively, and the accession numbers were XP_012649548 and BBMII III04695.The sequence alignment of the two genes was carried out by software APE, and the sequence of the two genes was completely identical. This gene contains a total of 4443 BP and encodes 1 480 amino acids. The encoded protein has a molecular weight of 165.307 ku. it is a secretory protein with one signal peptide and four transmembrane regions. Conclusion the bioinformatics method can be used to predict the number of antigen epitopes in the coryloid proteins of Barb 茅 worm in voles, and it can be used as a candidate for vaccine and diagnostic antigens.
【作者单位】：中国疾病预防控制中心寄生虫病预防控制所卫生部寄生虫病原与媒介生物学重点实验室世界卫生组织热带病合作中心国家级热带病国际联合研究中心;
【基金】：公益性卫生行业科研专项(No.201202019) 上海市卫生计生委青年基金项目(No.20154Y0155)
【分类号】：R382;Q811.4

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