农杆菌介导的ZmRLK基因转化玉米的分析

发布时间：2018-03-22 01:24

本文选题：玉米　切入点：ZmRLK基因　出处：《分子植物育种》2017年01期 　论文类型：期刊论文

【摘要】：玉米是世界上非常重要的粮食作物,其产量受诸多生物胁迫及非生物胁迫的影响。转基因技术是玉米遗传改良的重要途径之一。利用转基因技术把抗逆基因导入玉米优良自交系,并使其后代具有抵抗逆境的能力,减少损害,从而实现高产稳产。本研究通过将抗冷基因ZmRLK在玉米自交系Y423中遗传转化,得到过量表达ZmRLK基因的抗冷玉米新种质。结果表明,以玉米自交系Y423幼胚为受体,经检测获得ZmRLK过表达阳性植株89株;干扰载体获得121株阳性植株;空载体获得31株阳性植株。转基因阳性植株的获得为培育转基因玉米新品种提供帮助。
[Abstract]:Corn is a very important food crop in the world. Transgenic technology is one of the important ways of genetic improvement in maize. The stress resistant gene is introduced into maize inbred lines by transgenic technology, and its offspring have the ability to resist stress. Through genetic transformation of cold resistant gene ZmRLK in maize inbred line Y423, a new cold resistant maize germplasm with over-expression of ZmRLK gene was obtained. The results showed that the immature embryo of maize inbred line Y423 was used as the receptor. 89 positive plants with ZmRLK overexpression, 121 positive plants with interference vector and 31 positive plants with empty vector were obtained.
【作者单位】：吉林大学植物科学学院;
【基金】：国家转基因生物新品种培育重大专项目(2014ZX0800305B) 吉林省科技厅项目(2013020611NY)共同资助
【分类号】：S513;Q943.2

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