矢竹地下茎转录组测序及节间生长相关基因表达分析

发布时间：2018-03-23 05:27

  本文选题：矢竹　切入点：转录组　出处：《南京林业大学学报(自然科学版)》2017年05期 　论文类型：期刊论文

【摘要】：【目的】揭示竹子地下茎生长发育的分子机制。【方法】利用高通量转录组测序对矢竹含不同发育阶段节的地下茎转录组图谱进行了分析研究,并利用qRT-PCR对节间生长相关基因进行表达模式分析。【结果】共获得了11 976条平均长度为1 008 bp的单基因簇(unigenes)。NCBI注释显示,63.8%的单基因簇具有注释结果,其中16 254条unigenes被注释到137个KEGG(京都基因与基因组百科全书)代谢通路中。Map Man分析共获得了1 864个转录因子及603个激素代谢及信号转导相关单基因簇。在功能基因方面,共获得564个与细胞壁构建相关的代谢基因,其中92个与木质素合成相关。此外,对9个与赤霉素、细胞壁合成及细胞骨架组织相关的基因,通过实时荧光定量PCR,分析了它们在节间不同发育阶段的表达模式。结果显示:细胞壁、细胞骨架下游功能相关基因在矢竹地下茎节间快速生长阶段表达最高;而赤霉素合成及信号传导相关基因则在节间伸长起始阶段最高。【结论】矢竹地下茎生长发育受到从各类激素、转录因子到其下游功能基因等组成的复杂分子网络的协同调控。
[Abstract]:[objective] to reveal the molecular mechanism of the growth and development of underground stems of bamboo. [methods] High-throughput transcriptome sequencing was used to analyze the transcriptional maps of underground stems with different development stages. The expression patterns of internode growth-related genes were analyzed by qRT-PCR. [results] A total of 11,976 single gene clusters with an average length of 1 008bp were obtained, indicating that 63.8% of the single gene clusters had annotated results. Of these, 16,254 unigenes were annotated into 137 KEGG (Kyoto Gene and Genome Encyclopedia) metabolic pathways. Map Man analysis obtained 1 864 transcription factors and 603 single gene clusters related to hormone metabolism and signal transduction. A total of 564 metabolic genes related to cell wall construction were obtained, of which 92 were related to lignin synthesis. In addition, 9 genes related to gibberellin, cell wall synthesis and cytoskeleton tissue were identified. The expression patterns of PCRs in different stages of internode development were analyzed by real-time fluorescence quantitative PCR.The results showed that the expression of genes related to the downstream function of cytoskeleton was the highest in the rapid growth stage of Internode of sagittal bamboo. The genes related to gibberellin synthesis and signal transduction were the highest at the beginning of Internode elongation. [conclusion] the growth and development of rhizome of sagittal bamboo is controlled by a complex molecular network composed of hormones, transcription factors and its downstream functional genes.
【作者单位】： 南方现代林业协同创新中心南京林业大学竹类研究所;
【基金】：国家林业公益性行业科研专项项目(201504106) 国家自然科学基金项目(31301808,31670602) 江苏高校优势学科建设工程资助项目(PAPD)
【分类号】：S795
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本文编号：1652089