华癸根瘤菌7653R hfq基因突变株的构建及其生物学特性

发布时间：2018-03-24 04:06

  本文选题：华癸中慢生根瘤菌R　切入点：hfq基因　出处：《微生物学报》2017年01期

【摘要】：【目的】研究hfq基因在Mesorhizobium huakuii 7653R抵抗外界不利环境和共生固氮中的功能特性。【方法】利用pK19mob同源重组方法构建7653R hfq基因的插入失活突变株7653RΔhfq,并构建互补菌株7653RΔhfq-C,对hfq在压力胁迫和共生固氮中的功能特性进行研究。【结果】与野生型7653R相比,突变株7653RΔhfq的生长速率降低,热激处理后致死率升高;hfq突变影响了7653R中部分sRNA的表达;在4.5%乙醇和50 mmol H_2O_2生长胁迫下,突变株适应性明显较野生型差。另外,接种突变株的紫云英结瘤能力和固氮酶活性都明显降低。【结论】hfq基因作为重要的转录后调控因子,在7653R抵御外界胁迫环境和与宿主紫云英的共生固氮中发挥了重要作用。
[Abstract]:[objective] to study the functional characteristics of hfq gene in Mesorhizobium huakuii 7653R resistance to external adverse environment and symbiotic nitrogen fixation. [methods] the insertion inactivated mutant 7653R 螖 hfq-Cof 7653R hfq gene was constructed by pK19mob homologous recombination method, and the complementary strain 7653R 螖 hfq-Cwas constructed. The functional characteristics in stress stress and symbiotic nitrogen fixation were studied. [results] compared with wild type 7653R, The growth rate of the mutant 7653R 螖 hfq decreased, the mortality increased after heat shock treatment, and the expression of some sRNA in 7653R was affected by the mutation. Under 4.5% ethanol and 50 mmol H_2O_2 growth stress, the adaptability of the mutant was significantly worse than that of the wild type. [conclusion] as an important posttranscriptional regulator, hfq gene plays an important role in the resistance to external stress and the symbiotic nitrogen fixation with host Ziyun. [conclusion] as an important posttranscriptional regulator, hfq gene plays an important role in resistance to external stress and symbiotic nitrogen fixation.
【作者单位】： 农业微生物学国家重点实验室 华中农业大学;
【基金】：国家自然科学基金(31371549,31460056)~~
【分类号】：Q939.114
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本文编号：1656617