刺参4个功能基因的表达分析

发布时间：2018-03-24 16:39

本文选题：刺参　切入点：腱生蛋白基因　出处：《大连海洋大学学报》2017年03期

【摘要】：为研究刺参Apostichopus japonicus的生长发育,采用实时定量PCR方法,对刺参腱生蛋白基因、胶原蛋白α2基因、整合蛋白αV基因和整合蛋白βL基因等4个候选功能基因不同月龄的组织表达进行了分析。结果表明:刺参12、13、14月龄时,腱生蛋白基因在刺参肠、管足、呼吸树、皮肤、体壁、体腔液细胞、疣足和纵肌等8个组织中均有不同程度的表达,其中在体腔液细胞和呼吸树组织中的表达量较高(P0.05);胶原蛋白α2基因在刺参管足、呼吸树、体壁、疣足和纵肌组织中的表达量随月龄的增加而逐渐增加,其中在呼吸树组织中表达量最高,在纵肌、疣足、体壁、肠和体腔液细胞等组织中的表达量逐渐降低;整合蛋白αV基因在纵肌组织中的表达量随月龄的增加而逐渐增加;整合蛋白βL基因在皮肤、体壁、疣足和管足组织中的表达量较低。本研究结果为深入研究刺参这4个功能基因提供了参考。
[Abstract]:In order to study the growth and development of Apostichopus japonicus, the tendon protein gene and collagen 伪 2 gene were analyzed by real-time quantitative PCR. Four candidate functional genes, integrin 伪 V gene and integrin 尾 L gene, were analyzed in tissues of different months of age. The results showed that at 14 months of age, the tendon protein gene was found in the intestine, tubules, respiratory tree, skin and body wall of Acanthopsis japonicus. There were different degrees of expression in 8 tissues, such as somatic fluid cells, verruca foot and longitudinal muscle, among which, the expression of collagen 伪 2 gene was higher in somatic fluid cells and respiratory trees, and collagen 伪 2 gene was found in the foot, respiratory tree and body wall of Acanthophora japonicus. The expression level of verruca foot and longitudinalis muscle increased gradually with the increase of age, the highest expression was found in respiratory tree, and the expression level in longitudinal muscle, verruca foot, body wall, intestinal and lumen fluid cells decreased gradually. The expression of integrin 伪 V gene in longitudinal muscle increased with the increase of age, and integrin 尾 L gene was expressed in skin and body wall. The results of this study provide a reference for the further study of the four functional genes of Acanthophora japonicus.
【作者单位】：大连海洋大学水产与生命学院;大连市水产产业技术创新联合会;
【基金】：国家自然科学基金资助项目(31572608) 辽宁省高等学校优秀人才支持计划项目(LR2014022) 大连市科技计划项目(2012B11NC049)
【分类号】：S917.4

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