电针足三里、中脘对M受体基因敲除小鼠胃运动的时效差异研究

发布时间：2018-03-26 04:12

本文选题：电针　切入点：胃运动　出处：《时珍国医国药》2017年03期

【摘要】：目的观察电针足三里、中脘调节小鼠胃运动的时效差异,探讨M_2、M_3受体在该时效差异中的作用异同。方法C57/BL6野生型小鼠9只,M_2受体基因敲除小鼠、M_(23)受体基因敲除小鼠各8只,利用自制水囊测压探头监测胃内压,待小鼠胃运动稳定后,分别电针足三里、中脘两穴各1min,(2m A,2/15Hz)。观察并记录电针前后的胃内压,并分析其数值变化。结果电针B6小鼠足三里,对胃运动具有即时兴奋效应;电针中脘对胃运动具有即时抑制效应及电针后抑制效应;电针M_2~(-/-)、M_(23)~(-/-)小鼠足三里,即时兴奋效应消失;电针M_(23)~(-/-)小鼠足三里,出现胃运动的电针后抑制效应;电针M_2~(-/-)小鼠中脘,对胃运动具有即时抑制效应及电针后抑制效应;电针M_(23)~(-/-)小鼠中脘,仅留有电针后抑制效应;M_(23)~(-/-)小鼠较B6小鼠,足三里电针后效应有明显改变,M_(23)~(-/-)小鼠较B6小鼠,中脘即时效应有明显改变,以上差异均具有统计学意义,均P0.05。结论电针足三里、中脘调节小鼠胃运动,存在效应方向和时效的双重差异;M_2、M_3受体机制与其效应差异密切相关。
[Abstract]:Objective to observe the effect of electroacupuncture in Zusanli (Zusanli) and Zhongwan (Zhongwan) on regulating gastric motility in mice, and to explore the effect of M2M _ 3 receptor on gastric motility in mice. Methods 9 mice of C57/BL6 wild-type mice with M2M _ 2 receptor gene knockout mice and 8 mice of M _ 2 receptor gene knockout mice were studied. The intragastric pressure was monitored by a self-made water bag manometry probe. After the gastric motility of the mice was stabilized, the gastric pressure was observed and recorded before and after electroacupuncture at Zusanli, Zhongwan and Zusanli, respectively. The intragastric pressure before and after electroacupuncture was observed and recorded, and the changes of its numerical value were analyzed. It has an immediate excitatory effect on gastric motility; an immediate inhibitory effect on gastric motility by electroacupuncture in Zhongwan and an inhibition effect after electroacupuncture; an electroacupuncture on M 2J / / M + MJ / M + + / M + M / M / M + + mice, and an instant excitatory effect disappeared in Zusanli mice; and an electroacupuncture on M ~ (23 / 23 / -) mouse Zusanli (Zusanli). The inhibitory effect of electroacupuncture on gastric motility; the inhibitory effect of electroacupuncture on gastric motility in M _ 2 / r _ (-) mice and after electroacupuncture; and the inhibitory effect of electroacupuncture on gastric motility in M _ (23) / -r _ (-) mice was only left after electroacupuncture in mice than in B6 mice. After electroacupuncture, the effect of Zusanli electroacupuncture was obviously changed. Compared with B6 mice, the immediate effect of Zusanli electroacupuncture was significantly different from that of B6 mice, and the above differences were all statistically significant (P0.05.Conclusion electroacupuncture in Zusanli, Zhongwan regulates gastric motility in mice. There is a double difference between the direction of effect and the time of effect. The mechanism of M2M3 receptor is closely related to the difference of effect.
【作者单位】：南京中医药大学针药结合教育部重点实验室;
【基金】：国家自然科学基金资助项目(No.81373749,No.81574071,No.81673883)
【分类号】：R245.97

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