辣椒轻斑驳病毒凤城分离物的鉴定、全基因测序及系统进化分析

发布时间：2018-03-26 08:58

本文选题：辣椒轻斑驳病毒　切入点：RT-PCR　出处：《植物保护》2017年02期

【摘要】：辣椒轻斑驳病毒Pepper mild mottle virus(PMMoV)属于烟草花叶病毒属,是辣椒上重要的病原病毒之一,近年来PMMoV对辽宁部分辣椒产区造成严重危害。本文采用DAS-ELISA检测以及RT-PCR的方法首次从辽宁省凤城市蔬菜产区辣椒病叶中检测出PMMoV,暂命名为PMMoV-FC。设计3对特异性引物对其进行扩增并测序后得到该病毒分离物全基因序列。将PMMoV-FC的全基因序列与已报道的国内外9个PMMoV分离物进行同源性分析,结果表明,其同源性介于94.3%~99.7%之间。基于全基因序列的系统发育进化分析表明,PMMoV-FC与国内分离物、日本分离物以及美洲分离物亲缘关系密切,而与韩国和西班牙分离物亲缘关系稍远。鉴于该病毒在辣椒上造成的严重危害,对于PMMoV-FC在辽宁地区的发生以及防治仍需进行更为详细的研究。
[Abstract]:Capsicum light mottle virus (Pepper mild mottle virusPMMoV) belongs to the genus Tobacco Mosaic virus, and is one of the most important pathogenic viruses in capsicum. In recent years, PMMoV has caused serious harm to some pepper producing areas in Liaoning Province. In this paper, DAS-ELISA detection and RT-PCR were used to detect PMMoV from pepper leaf of Fengcheng vegetable growing area of Liaoning Province for the first time, tentatively named PMMoV-FC. three pairs of specific primers were designed. The whole gene sequence of the virus isolate was obtained by amplification and sequencing. The whole gene sequence of PMMoV-FC was homologous to 9 PMMoV isolates reported at home and abroad. The results showed that the homology of PMMoV-FC was between 94.3% and 99.7%. Phylogenetic analysis based on the whole gene sequence showed that PMMoV-FC was closely related to domestic isolates, Japanese isolates and American isolates. In view of the serious harm caused by the virus on pepper, the occurrence and prevention of PMMoV-FC in Liaoning should be studied in more detail.
【作者单位】：沈阳农业大学植物保护学院;
【基金】：公益性行业(农业)科研专项(201303028) 沈阳农业大学2016大学生创新训练计划项目
【分类号】：S436.418

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