水稻斑马叶突变体zebra1349的表型鉴定及基因精细定位

发布时间：2018-03-27 09:03

本文选题：水稻(Oryza　切入点：sativa　出处：《作物学报》2016年07期

【摘要】：从恢复系育种材料[R128//(R318/R1025)F1]F6中获得一个新的斑马叶突变体zebra1349,突变体秧苗期如果不移栽,与野生型一样表现绿色,移栽后5 d新抽出的叶片包括叶鞘会呈现出与叶脉垂直的黄绿相间的条纹,移栽后30 d抽出的叶片又表现正常绿色,成熟期主要农艺性状与野生型无明显差异。与野生型相比,突变体六叶期斑马叶黄区部位的总叶绿素、叶绿素a、叶绿素b和类胡萝卜素的含量分别下降了55.86%、61.02%、39.34%和47.03%。透射电镜(TEM)观察表明,突变体斑马叶绿区部位叶绿体发育正常;黄区部位叶肉细胞中叶绿体结构异常,类囊体膜退化和分解严重,类囊体基粒片层数量明显减少,片层间距拉大,排列疏松。对zebra1349与正常叶色品种杂交F1、F2代的遗传分析表明该性状受1对隐性核基因调控。利用1192株zebra1349/02428 F2隐性定位群体,最终把ZEBRA1349基因定位在水稻第12染色体In Del标记indel39和indel44之间,其遗传距离分别为0.04 c M和0.17 c M,根据日本晴基因组序列推测,两标记之间的物理距离约为89 kb。本研究为ZEBRA1349基因的图位克隆和功能研究以及分子标记辅助育种奠定了基础。
[Abstract]:A new zebra1349 zebra1349 mutant was obtained from restorer line [R128//(R318/R1025)F1] F6. Five days after transplanting, the newly extracted leaves, including leaf sheaths, showed yellow and green stripes perpendicular to the veins, and the leaves extracted 30 days after transplanting showed normal green, and there was no significant difference between the main agronomic characters and the wild type in the mature stage, compared with the wild type. The contents of total chlorophyll, chlorophyll a, chlorophyll b and carotenoid in the leaves of zebra at the six-leaf stage decreased by 55.86%, 61.02% and 47.03%, respectively. The observation of transmission electron microscopy (TEM) showed that the chloroplast developed normally in the green area of zebra leaves. The chloroplast structure of mesophyll cells in the yellow region was abnormal, thylakoid membrane degraded and decomposed seriously, the number of thylakoid granulocyte lamellae decreased obviously, and the interlamellar spacing was enlarged. The genetic analysis of F _ 1 F _ 2 generation of zebra1349 and normal leaf color variety showed that the trait was regulated by a pair of recessive nuclear genes. 1192 zebra1349/02428 F _ 2 recessive loci were used. Finally, the ZEBRA1349 gene was mapped between in Del marker indel39 and indel44 on rice chromosome 12, with genetic distances of 0.04 cm and 0.17 cm, respectively. The physical distance between the two markers is about 89 kb. This study laid a foundation for map cloning and functional study of ZEBRA1349 gene and molecular marker-assisted breeding.
【作者单位】：湖南农业大学农学院;湖南杂交水稻研究中心杂交水稻国家重点实验室;衡阳市农业科学研究所;
【基金】：国家高技术研究发展计划(863计划)项目“强优势水稻杂交种的创制与应用”(2011AA10A101) 衡阳市科技局重点项目“黄色带状标记基因在两用核不育系中的利用”(2011KZ15)资助 supported by the grants from National High-tech R&D Program of China(863 Program)(2011AA10A101) the Key Project Funded by the Hengyang Science and Technology Bureau(2011KZ15)
【分类号】：S511

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