玉米磷转运蛋白基因ZmPht3;1的克隆和功能分析

发布时间：2018-03-27 13:39

  本文选题：玉米　切入点：磷转运蛋白　出处：《生物技术通报》2016年12期

【摘要】：Pht3(phosphate transporter 3)磷转运子家族属于一类低亲和力磷转运蛋白,在调节植株体内磷素的动态平衡中发挥重要作用。为了初步探讨玉米中Zm Pht3;1基因的结构特征及其磷饥饿的响应机制,利用同源克隆的方法从耐低磷玉米自交系Mo17中分离得到Zm Pht3;1基因,并运用实时荧光定量PCR和亚细胞定位的方法对其进行深入研究。结果表明,Zm Pht3;1的编码区全长1 101 bp,编码366个氨基酸,含有典型的线粒体转运家族(mitochondrial carrier family,MCF)结构特征与6个疏水跨膜结构。荧光定量PCR分析表明,该基因在两个极端材料的根系与叶片中均有表达,而表达模式差异显著,在耐低磷玉米自交系Mo17的根系和叶片中表现为缺磷胁迫前期的一般性反应和后期的特异性反应。转化烟草的亚细胞定位结果显示,Zm Pht3;1主要分布于细胞膜上,可能是一个双亲和转运体,在玉米响应磷饥饿胁迫过程中发挥重要的适应性调节作用。
[Abstract]:Pht3(phosphate transporter 3) P transporter family belongs to a low affinity phosphorous transporter family, which plays an important role in regulating the dynamic balance of phosphorus in plants. The Zm Pht3H1 gene was isolated from low phosphorus tolerant maize inbred line Mo17 by homologous cloning. The method of real-time fluorescence quantitative PCR and subcellular localization was used to study it. The results showed that the coding region of Zm Pht3H1 was 1 101 BP, encoding 366 amino acids. There are typical mitochondrial carrier family MCFs and six hydrophobic transmembrane structures. Fluorescence quantitative PCR analysis showed that the gene was expressed in both the roots and leaves of the two extreme materials, and the expression patterns were significantly different. In the roots and leaves of low phosphorus tolerant maize inbred line Mo17, the general and late specific responses were observed in the early stage of phosphorus deficiency stress and the specific response in the later stage. The subcellular localization results showed that Zm Pht3O1 was mainly distributed on the cell membrane. It may be a parent and transporter, which plays an important role in the adaptive regulation of maize response to phosphorus starvation stress.
【作者单位】： 成都农业科技职业学院;成都丹凤科技有限公司;
【分类号】：S513;Q943.2
，

本文编号：1671745