转基因植物乳杆菌对雌性大鼠生殖发育的影响

发布时间：2018-03-28 04:23

本文选题：转基因微生物　切入点：多代实验　出处：《广东医学》2017年05期

【摘要】：目的探讨转基因植物乳杆菌对连续传代雌性大鼠生殖发育的影响。方法将雌雄SD大鼠分组,6只雌性一笼,6只雄性一笼。对照组与实验组,分别为一笼雌和一笼雄。对4周龄SD大鼠进行灌胃,对照组予以非转基因植物乳杆菌,实验组予以转基因植物乳杆菌,连续灌胃4周后进行雌雄配对,对子一代、子二代、子三代进行同样的灌胃实验8周。比较两组雌性大鼠不同时间点身长、尾长、体质量、肛殖距、卵巢质量、激素水平。结果对照组与实验组子一代、子二代、子三代雌性大鼠灌胃同时间点比较,身长、尾长、体质量、肛殖距差异无统计学意义(P0.05)。无论是对照组还是实验组,灌胃同时间点子一代、子二代、子三代雌性大鼠身长、尾长、体质量、肛殖距均有不同程度的增加,但差异无统计学意义(P0.05)。子一代、子二代、子三代雌性大鼠比较,对照组与实验组初级卵泡、次级卵泡、黄体计数、雌二醇、孕酮、阴道开口时间差异无统计学意义(P0.05)。无论是对照组还是实验组,子一代、子二代、子三代雌性大鼠初级卵泡、次级卵泡、黄体计数、雌二醇、孕酮均有不同程度的增加,阴道开口时间均有不同程度的减少,但差异无统计学意义(P0.05)。结论转基因植物乳杆菌对连续传代雌性大鼠生殖发育无明显影响。
[Abstract]:Objective to investigate the effect of transgenic Lactobacillus plantarum on the reproductive development of female rats. Methods male and female SD rats were divided into 6 female and 6 male cages. A cage female and a cage male were perfused into the stomach of 4-week-old SD rats, the control group was given non-transgenic Lactobacillus plantarum, and the experimental group was given transgenic Lactobacillus plantarum. The third generation was given the same gastric perfusion experiment for 8 weeks. The body length, tail length, body mass, anal space, ovarian mass, hormone level of the two groups were compared at different time points. Results the control group and the experimental group were divided into two groups: the control group and the experimental group. There was no significant difference in body length, tail length, body mass and anal colonization distance between the third generation female rats and the third generation female rats at the same time points. Whether the control group or the experimental group, the female rats of the same time point generation, the second generation, the third generation, the female rats of the third generation had the same body length and tail length, both in the control group and in the experimental group. The body mass and anal distance increased in different degrees, but the difference was not statistically significant (P 0.05). The primary follicle, secondary follicle, luteal body count, estradiol, progesterone were compared between the control group and the experimental group, compared with the female rats of the first generation, the second generation, the third generation, the control group and the experimental group. There was no significant difference in vaginal opening time between control group and experimental group. The number of primary follicle, secondary follicle, luteal body, estradiol and progesterone increased in different degrees in the control group and the experimental group, and the primary follicles, secondary follicles, luteal body count, estradiol and progesterone in the female rats of the second generation, the second generation and the third generation. The vaginal opening time was decreased to some extent, but the difference was not statistically significant (P 0.05). Conclusion there is no significant effect of Lactobacillus plantarum transgenic on the reproductive development of continuous subculture female rats.
【作者单位】：华北理工大学公共卫生学院;华北理工大学生命科学学院;
【基金】：国家自然科学基金资助项目(编号:31501586) 河北省自然科学基金资助项目(编号:C2015209288)
【分类号】：R114

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