信号物质对小麦菌根中氮磷转运蛋白基因表达的调控作用研究

发布时间：2018-03-28 05:20

本文选题：信号物质　切入点：小麦　出处：《西北农林科技大学》2017年硕士论文

【摘要】：前期研究表明,丛枝菌根(AM)真菌侵染能够调控小麦菌根中氮、磷转运蛋白基因的表达,但是具体调控机制还不清楚。本论文通过设计分根分室盆栽试验,使用实时荧光定量PCR技术精确定量了小麦菌根中磷转运蛋白(PT)基因,硝态氮转运蛋白(NRT)基因或铵态氮转运蛋白(AMT)基因的表达水平,研究了AM真菌根外菌丝传递的氮磷养分信号和菌根共生信号等两类信号物质对小麦菌根中氮、磷转运蛋白基因表达的调控作用,主要的研究结果如下:1.不论根外菌丝能否向宿主植物输送氮、磷养分,只要接种丛枝菌根就能够下调1个磷饥饿诱导的磷转运蛋白基因TaPHT1.2及3个NRT基因的表达(TaPHT1.2、TaNRT2.1和TaNRT2.3),与AM真菌根外菌丝是否有氮磷养分传递无关;而小麦菌根中TaPT4、TaNRT2.2和TaAMT1.2等3个基因的表达则受AM真菌根外菌丝传递的氮、磷养分信号的下调。2.菌根共生信号物质独脚金内酯、类黄酮或溶血卵磷脂能够下调4个硝态氮转运蛋白基因的表达(TaNRT1.1、TaNRT1.2、TaNRT2.1和TaNRT2.3);菌根因子处理可显著下调TaNRT1.2和TaNRT2.1的基因表达水平。独脚金内酯、类黄酮和溶血卵磷脂等3类共生信号均未影响小麦菌根内4个磷转运蛋白基因(TaPT10,TaPT11,TaPHT1.2和TaPT4)的表达;菌根因子处理(Myc-F.m)能够显著上调1个AM诱导型磷转运蛋白基因(TaPT10)的表达,而对其他磷转运蛋白基因的表达没有显著影响。
[Abstract]:Previous studies have shown that arbuscular mycorrhizal fungi infection can regulate the expression of nitrogen and phosphorus transporter genes in wheat mycorrhiza, but the specific regulatory mechanism is not clear. The expression levels of phosphorous transporter (PTT) gene, nitrate nitrogen transporter gene (NRTT) gene and ammonium nitrogen transporter (AMT) gene in wheat mycorrhiza were determined by real-time fluorescence quantitative PCR. The regulation of N and P transporter gene expression in wheat mycorrhizal mycorrhiza by N P nutrient signal and mycorrhizal symbiosis signal transmitted by mycelium of AM fungi was studied. The main results are as follows: 1. Whether or not the hyphae can transport nitrogen and phosphorus nutrients to the host plant, The expression of one phosphorous transporter gene TaPHT1.2 and three NRT genes induced by phosphorus starvation could be down-regulated by inoculation of arbuscular mycorrhizal mycorrhiza. The expression of TaPHT1.2, TaNRT2.1 and TaNRT2.3 was not related to the transfer of nitrogen and phosphorus nutrients in mycelium of AM fungi. However, the expression of three genes, TaPT4, TaPT4, TaNRT2.2 and TaAMT1.2, was down-regulated by the signal of nitrogen and phosphorus from mycorrhizal mycelia of AM fungi. The symbiotic signal substance of mycorrhizal symbiotic signal, goldenolactone, was observed. Flavonoids or lysophosphatidylcholine could down-regulate the expression of four nitrate nitrogen transporter genes, TaNRT1.1, TaNRT1.2, TaNRT2.1 and TaNRT2.3, and mycorrhizal factor treatment could significantly down-regulate the gene expression levels of TaNRT1.2 and TaNRT2.1. The symbiotic signals of flavonoids and lysophosphatidylcholine did not affect the expression of four phosphorous transporter genes in wheat mycorrhiza, TaPT10, TaPT11, TaPHT1.2 and TaPT4, and mycorrhizal factor treatment (Myc-F.m) could significantly up-regulate the expression of an AM inducible phosphorous transporter gene, TaPT10). However, there was no significant effect on the expression of other phosphorous transporter genes.
【学位授予单位】：西北农林科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S512.1

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