房颤患者Cx40基因单核苷酸多态性研究

发布时间：2018-03-28 10:36

本文选题：GJA基因　切入点：rs　出处：《中国现代医学杂志》2017年19期

【摘要】：目的探讨缝隙连接蛋白40基因(GJA5)单核苷酸多态性在房颤发生中的作用。方法选择2012年6~12月南华大学附属第一医院心血管内科住院患者200例,分为房颤组98例(持续性发颤)及对照组102例(窦性心律)。收集患者一般临床资料并通过经胸心脏彩超采集患者左房内径值,静脉采血提取DNA,经聚合酶链反应(PCR)及电泳法检测SNP的位点,PCR扩增产物纯化后直接测序查看SNP位点;哈德温伯格平衡检验后,比较各组间基因分型和基因频率,就房颤危险因素非条件Logistic回归分析。结果 GJA5基因rs10465885存在A/G多态性,房颤组等位基因A、G频率分别为0.54和0.46。对照组等位基因A、G频率分别为0.45和0.55。哈德温伯格平衡检验显示组间均符合其平衡,房颤组AA基因型高于对照组,AG、GG基因型以及等位基因频率差异无统计学意义。GJA5基因(rs10465885)AA基因型(OR∧=2.49)和左房直径(OR∧=1.29)是房颤的危险因素,GJA5基因(rs10465885)基因型和左房直径无关。结论 GJA5基因rs10465885位点存在基因多态性,AA基因型与左房直径是房颤的危险因素。
[Abstract]:Objective to investigate the expression of connexin 40 gene (GJA5) of single nucleotide polymorphisms in the occurrence of atrial fibrillation in 2012. Methods 6~12 Department of Cardiology First Hospital Affiliated to Nanhua University inpatients 200 cases, divided into 98 cases of atrial fibrillation group (continuous shaking) and 102 cases of the control group (sinus rhythm) were collected. The clinical data and through transthoracic echocardiography in patients with left atrial diameter collection value, venous blood DNA was extracted by polymerase chain reaction (PCR) detection and SNP electrophoresis sites, PCR amplification products were purified and directly sequenced to view the SNP site; the hardy Weinberg equilibrium test, comparisons between groups of genotype and gene frequency, on the risk factors of non atrial fibrillation conditional Logistic regression analysis. Results the GJA5 gene rs10465885 A/G polymorphism, AF group allele frequencies were 0.54 A, G and 0.46. control group A allele frequencies were 0.45, G and 0.55. Bernhard Weinberg equilibrium test group are in line with its equilibrium, AF group of AA genotype was higher than the control group, AG, no statistically significant difference between GG genotype and allele frequency of.GJA5 gene (rs10465885) AA genotype (OR ^ =2.49) and left atrial diameter (OR ^ =1.29) is a risk factor for atrial fibrillation. GJA5 gene (rs10465885) genotype and left atrial diameter. Conclusion GJA5 gene polymorphism rs10465885 locus, AA genotype and the left atrial diameter is a risk factor for atrial fibrillation.

【作者单位】：南华大学附属第一医院;
【基金】：国家自然科学基金(No:30900625)
【分类号】：R541.75

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