岩藻糖基转移酶2基因多态性与新疆地区哈萨克族、汉族胃腺癌发病的关系

发布时间：2018-03-29 06:32

本文选题：胃腺癌　切入点：岩藻糖基转移酶　出处：《山东医药》2017年10期

【摘要】：目的探讨岩藻糖基转移酶2(FUT2)基因多态性与新疆地区哈萨克族、汉族胃腺癌发病的关系。方法选取胃腺癌患者299例(病例组)、体检健康者333例(对照组),病例组中汉族167例、哈萨克族132例,对照组中汉族182例、哈萨克族151例。取清晨全血,采用PCR和DNA测序相结合的方法检测PUT2基因G739A、A385T、C375T位点的单核苷酸多态性。收集患者临床资料,采用多因素Logistic回归分析各因素与胃腺癌的关系。结果病例组和对照组汉族人群G739A位点AA基因分别为57、27例(P0.01),哈萨克族人群G739A位点AA基因型分别为32、18例(P0.01),携带G739A位点A等位基因的汉族、哈萨克族个体分别是相对应民族非携带者患胃腺癌风险的1.655倍(95%CI为1.227~2.233,P=0.001)和1.430倍(95%CI为1.026~1.992,P=0.035);A385T位点TT基因型在汉族及哈萨克族分别为7、12例,显著高于相同民族对照组的1、3例(P均0.05);C357T位点T等位基因在汉族及哈萨克族病例组和对照组中的差异均无统计学意义(P均0.05)。病例组中汉族和哈萨克族A385T位点T等位基因分别为39、45例(P0.01),对照组中两民族G739A、A385T、C357T位点基因型及等位基因比较差异均无统计学意义(P均0.05)。多因素回归分析显示,携带G739A位点A等位基因且现在还在吸烟或饮酒者或幽门螺杆菌(Hp)阳性者,患胃腺癌风险增加2.035、2.360、3.815倍。结论新疆地区汉族、哈萨克族胃腺癌的发生与FUT2基因G739A、A385T位点多态性有关,且胃腺癌患者中哈萨克族A385T位点突变频率高于汉族,同时吸烟、饮酒、Hp感染亦可增加携带G739A位点A等位基因者胃腺癌的患病风险。
[Abstract]:Objective to investigate the relationship between the polymorphism of fucoidyltransferase 2FUT2 gene and the incidence of gastric adenocarcinoma in Kazak and Han nationality in Xinjiang. Methods 299 patients with gastric adenocarcinoma (case group) and 333 healthy persons (control group, Han nationality, 167 cases) were selected. 132 Kazakh patients, 182 Han nationality and 151 Kazakh patients were enrolled in this study. The single nucleotide polymorphisms of PUT2 gene G739AnA385TnC375T were detected by PCR and DNA sequencing in early morning blood samples, and the clinical data of the patients were collected. Multivariate Logistic regression analysis was used to analyze the relationship between the factors and gastric adenocarcinoma. Results the AA gene of G739A locus was 5739 A locus in the case group and the control group, and the AA genotype of G739A locus in Kazak population was 32or 18 cases with G739A locus, carrying G739A locus, respectively. A allele of the Han nationality, The risk of gastric adenocarcinoma in Kazakh individuals was 1.655 times higher than that in non-carriers (CI = 1.2272.233P0. 001) and 1.430 times higher than that of controls. The TT genotype at the A385T locus in Han nationality and Kazak nationality were 712, respectively. There was no significant difference in T alleles at C357T locus between Han and Kazakh patients compared with the control group (P < 0.05). The T allele of A385T locus in Han nationality and Kazak nationality was not significantly higher than that in the same ethnic control group (P < 0.05). There was no significant difference in the genotype and allele of G739An A385T C357T locus between the two ethnic groups (P < 0.05). The multivariate regression analysis showed that there was no significant difference between the two groups in the genotype and allele of G739AX A385T C357T locus (P < 0.05). The G739A allele A allele, which is still in smoking or drinking or Helicobacter pylori (HP) positive, increased the risk of gastric adenocarcinoma by 2.035n 2.360,3.815 fold. Conclusion the occurrence of gastric adenocarcinoma in Han and Kazak nationality in Xinjiang is related to the polymorphism of G739AH385T locus of FUT2 gene. Moreover, the mutation frequency of A385T locus in Kazak was higher than that in Han nationality, while smoking and drinking HP infection could increase the risk of gastric adenocarcinoma with G739A A allele.
【作者单位】：新疆维吾尔自治区人民医院;
【基金】：新疆维吾尔自治区自然科学基金资助项目(2014211A053)
【分类号】：R735.2

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