凤丹油体膜蛋白基因PoOLE17.5的克隆与表达特性分析

发布时间：2018-03-30 11:06

本文选题：凤丹　切入点：PoOLE.基因　出处：《分子植物育种》2017年08期

【摘要】：油体是油料作物种子储存脂肪的细胞器,油体膜蛋白是构成油体的重要组分,对维持油体大小和稳定起着重要作用。本研究以油体膜蛋白为对象,克隆了凤丹的油体膜蛋白基因(OLE),其cDNA全长783 bp,开放阅读框长507 bp,共编码169个氨基酸,蛋白的分子量为17.48 k D,故将该基因命名为PoOLE17.5。生物信息学分析显示,该基因编码的蛋白包含2个亲水性区域和1个疏水性区域,并具有1个保守结构域"Oleosin domain"。而后的表达模式显示,PoOLE17.5在凤丹种子发育过程中的表达水平逐渐上升,在种子接近成熟时表达水平最高,而后略有下降。这一结果有利于今后通过基因工程手段调控凤丹油体发育。
[Abstract]:Oil body is the organelle of oil plant seed to store fat. Oil body membrane protein is an important component of oil body and plays an important role in maintaining oil body size and stability. The oil body membrane protein (Ole) gene of Fengdan was cloned. Its cDNA length was 783bpand the length of open reading frame was 507bp. it encoded 169 amino acids, and the molecular weight of the protein was 17.48kD. therefore, the gene was named Poole 17.5. Bioinformatics analysis showed that, The protein encoded by the gene contains two hydrophilic and one hydrophobic regions, and has a conserved domain "Oleosin domain". The expression pattern shows that the expression level of PoOLE17.5 increases gradually during the seed development of Fengdan. The expression level was the highest in the seed near maturation, and then decreased slightly. This result is helpful to regulate the oil body development of Fengdan by genetic engineering in the future.
【作者单位】：扬州大学动物科学与技术学院;扬州大学园艺与植物保护学院;
【基金】：江苏省林业三新工程项目(LYSX[2016]45) 江苏省农业科技自主创新资金(CX(14)2135)共同资助
【分类号】：Q943.2;S565.9

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