番木瓜成熟衰老蛋白基因CpSSA的克隆与表达分析

发布时间：2018-03-30 13:30

本文选题：番木瓜　切入点：衰老相关蛋白　出处：《分子植物育种》2017年04期

【摘要】：衰老相关蛋白(senescence-associated protein,SSA)基因在果实成熟衰老中起重要作用。为了探究番木瓜衰老相关蛋白基因,以‘大白八号’番木瓜果实为材料,采用RACE技术扩增出番木瓜SSA基因全长cDNA序列,命名为CpSSA(登录号:KX885408)。生物信息学分析显示,CpSSA全长1 203 bp,包含一个420 bp的开放性阅读框。番木瓜SSA蛋白含有139个氨基酸,分子质量为15.172 4 kD,等电点为9.61。该蛋白的氨基酸序列与桃、葡萄、杨树、菠菜的氨基酸序列有较高的同源性。系统进化树显示,番木瓜SSA与葡萄SSA表现出最小的进化距离,亲缘关系最接近。用荧光定量PCR分析在不同处理不同成熟度番木瓜果实中CpSSA基因的表达差异,结果显示在乙烯处理后的6 h和12 h表达量显著升高,在1-MCP处理中表达量较低,在乙烯/1-MCP处理果实中的表达模式与成熟衰老有一定相关性,推测CpSSA基因可能参与了番木瓜果实的成熟衰老进程。
[Abstract]:Senescence-associated protein (SSA) gene plays an important role in fruit senescence. In order to investigate the senescence related protein gene of papaya, the full-length cDNA sequence of papaya SSA gene was amplified by RACE. CpSSA was named as CpSSA (accession number: KX885408). Bioinformatics analysis showed that CpSSA was 1 203 BP in length and contained a 420bp open reading frame. Papaya SSA protein contained 139 amino acids, molecular weight was 15.172 kD and isoelectric point was 9.61.The amino acid sequence of CpSSA was similar to that of peach. The amino acid sequences of grape, poplar and spinach have high homology. Phylogenetic tree shows that papaya SSA has the smallest evolutionary distance from grape SSA. The results showed that the expression of CpSSA gene was significantly increased at 6 and 12 hours after ethylene treatment, but low in 1-MCP treatment. The expression pattern of ethylene / 1-MCP was correlated with maturation and senescence, which suggested that CpSSA gene might be involved in the ripening and senescence process of papaya fruit.
【作者单位】：福建农林大学园艺学院;福建农林大学园艺植物遗传育种研究所;
【基金】：福建农林大学园艺学院青年学术骨干培养基金项目(FAFU2012YYPY05)资助
【分类号】：Q943.2;S667.9

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