POU1F1基因多态性与藏羊生长性状的关联性分析

发布时间：2018-03-30 17:33

本文选题：藏羊　切入点：POUF基因　出处：《西北农林科技大学学报(自然科学版)》2017年09期

【摘要】：【目的】探讨POU1F1基因的多态性与藏羊生长性状之间的关联性,寻找与藏羊生长性状相关的分子标记。【方法】利用DNA测序方法检测藏羊POU1F1基因的多态位点并对其进行基因分型,分析单一多态位点不同基因型及双倍型与藏羊生长性状的关联性。【结果】在POU1F1基因第4内含子上检测出1个多态位点g.14205GA,在第5内含子上检测出2个多态位点g.15223GA和g.15286AG。关联性分析表明,g.14205GA位点上AA基因型的体质量和体长分别极显著(P0.01)和显著(P0.05)高于GG和AG基因型;g.15223GA位点上GG基因型的体质量显著高于GA基因型(P0.05),极显著高于AA基因型(P0.01);g.15286AG位点AA基因型体质量极显著高于GG基因型(P0.01),体高显著高于GG基因型(P0.05)。单倍型Hap7(-GGA-)的发生频率最高,达到42.40%,其次为单倍型Hap5(-GAA-)和单倍型Hap3(-AGA-),发生频率分别为21.50%和14.70%。此外,POU1F1的r2值均小于0.33,说明这3个多态位点之间不存在强的连锁性。通过合并基因型发现,双倍型H7H7(GGA-GGA)的各个生长性状表现最优。【结论】POU1F1基因的3个SNP位点可用于藏羊生长性状的选育。
[Abstract]:[objective] to investigate the association between the polymorphism of POU1F1 gene and the growth traits of Tibetan sheep, and to search for the molecular markers related to the growth traits of Tibetan sheep. [methods] the polymorphic loci of POU1F1 gene of Tibetan sheep were detected by DNA sequencing and genotyped. [results] one polymorphic locus (g.14205GA) was detected in intron 4 of POU1F1 gene, two polymorphic loci (g.15223GA) and two loci (g.15223GA) were detected in intron 5 of POU1F1 gene. The correlation analysis showed that the body weight and body length of AA genotype were significantly higher than those of GG and AG genotypes at GG and AG loci, respectively, and the body weight and body length of AA genotypes were significantly higher than those of GA genotypes (P0.05A, P 0.05). The body weight of AA genotype was significantly higher than that of GG genotype P0.01G, and the body height was significantly higher than that of GG genotype (P0.05). Haplotype Hap7G GGA-had the highest frequency of occurrence. The frequency of occurrence was 21.50% and 14.70%, respectively. In addition, the r2 values of POU1F1 were all less than 0.33, indicating that there was no strong linkage among the three polymorphic loci. [conclusion] three SNP loci of POU1F1 gene can be used for breeding growth traits of Tibetan sheep.
【作者单位】：渭南职业技术学院农学院;渭南畜牧兽医局;
【基金】：渭南职业技术学院院级重点课题(WZYZ201704)
【分类号】：S826

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