大豆GmCOL1和GmCOL13启动子和基因组基因的克隆与生物信息学分析

发布时间：2018-03-31 10:41

  本文选题：大豆　切入点：Gm　出处：《生物技术通报》2017年08期

【摘要】：以大豆天隆1号为材料提取基因组DNA,分别克隆到长2 631 bp的Gm COL1启动子和2 809 bp的Gm COL13启动子。此外,还克隆到Gm COL1和Gm COL13基因组基因长度分别为3 488 bp和2 798 bp,它们分别编码含348个和366个氨基酸的蛋白质。利用Plant CARE分析Gm COL1和Gm COL13的启动子序列发现,它们均含有以下调控元件:生物钟元件(Circadian)、光响应元件(ACEG-box等)、ABA响应元件(ABRE)、干旱诱导元件(MBS)、低温响应元件(LTR)。Prot Param分析表明,Gm COL1和Gm COL13蛋白质的分子量分别为38.47 k D和40.91 k D,并且都是亲水性蛋白。GOR分析显示,Gm COL1和Gm COL13的二级结构含有Alpha helix,Extended strand和Random coil。在线网站PROSITE的分析说明,Gm COL1和Gm COL13有两个Zinc finger B-box和一个CCT domain(CO,CO-LIKE and TOC1 domain)结构域。
[Abstract]:Genomic DNA was extracted from soybean Tianlong 1 and cloned into 2 631 BP GM COL1 promoter and 2 809 BP GM COL13 promoter, respectively. The genomic gene length of GM COL1 and GM COL13 were 3 488 BP and 2 798 BP, respectively, which encoded 348 and 366 amino acids, respectively. The promoter sequences of GM COL1 and GM COL13 were analyzed by Plant CARE. They all contain the following regulatory elements: circadian clock element, light response element ACEG-box, ACEG-box, drought induced element, and low temperature response element, LTR.Prot Param analysis shows that the molecular weight of Gm COL1 and GM COL13 protein are 38.47kD and 40.91, respectively. The molecular weight of Gm COL1 and GM COL13 protein are 38.47kD and 40.91, respectively. The molecular weight of Gm COL1 and GM COL13 protein are 38.47kD and 40.91, respectively. The secondary structures of Gm COL1 and GM COL13 contain Alpha helix extended strand and Random Coil.The analysis of PROSITE shows that Gm COL1 and GM COL13 have two Zinc finger B-box and one CCT domaine CO-CO-LIKE and TOC1 domain.
【作者单位】： 中国农业科学院作物科学研究所国家农作物基因资源与遗传改良重大科学工程农业部北京大豆生物学重点实验室;
【基金】：国家自然科学基金项目(31371703,31570289) 转基因专项(2016ZX08004-005,2016ZX08009001-001)
【分类号】：Q943.2;S565.1
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本文编号：1690381