赖氨酸二肽对奶牛乳腺mTOR信号通路元件基因表达的影响

发布时间：2018-04-01 14:31

本文选题：赖氨酸二肽　切入点：αs酪蛋白　出处：《西北农业学报》2017年07期

【摘要】：为研究赖氨酸二肽对奶牛乳腺组织中哺乳动物雷帕霉素靶蛋白(mTOR)信号通路的影响,在体外培养的奶牛乳腺组织培养液中分别添加赖氨酸—赖氨酸、赖氨酸—亮氨酸和赖氨酸—组氨酸二肽,等量取代培养液中φ=5%、5%和15%的游离赖氨酸(赖氨酸总质量浓度为210 mg/L);在此基础上,在赖氨酸组和赖氨酸—赖氨酸二肽5%取代组分别添加终浓度为0和100 nmol/L的mTOR抑制剂雷帕霉素。试验结束后收集乳腺组织用于αs1酪蛋白、mTOR、4E-BP1和S6K1的基因表达检测。结果发现,与游离氨基酸相比,添加3种赖氨酸二肽显著增加αs1酪蛋白、mTOR、4E-BP1和S6K1基因的表达;添加雷帕霉素抑制上述基因的表达,赖氨酸—赖氨酸二肽可以降低雷帕霉素的抑制作用。结果表明,除作为合成底物外,赖氨酸二肽还可能通过调控mTOR信号通路促进乳蛋白的合成。
[Abstract]:In order to study the effect of lysine dipeptide on the signaling pathway of rapamycin target protein mTORin in mammal mammary tissue of dairy cow, lysine-lysine was added to the culture medium of dairy cow mammary tissue in vitro.Lysine-leucine and lysine-histidine dipeptide were substituted for 5% and 15% free lysine in culture medium (total lysine concentration was 210 mg / L ~ (-1)).In the lysine group and the lysine-lysine dipeptide 5% substitution group, the final concentration of mTOR inhibitor rapamycin was 0 and 100 nmol/L, respectively.After the experiment, breast tissues were collected to detect the gene expression of 伪 S1 casein mTOR4E-BP1 and S6K1.The results showed that the addition of three lysine dipeptides significantly increased the expression of 伪 s1 casein mTOR4E-BP1 and S6K1 genes, and the addition of rapamycin inhibited the expression of these genes.Lysine-lysine dipeptide can reduce the inhibitory effect of rapamycin.The results showed that lysine dipeptide could promote the synthesis of lactoprotein by regulating the mTOR signaling pathway.
【作者单位】：山东潍坊科技学院动物科学研究所;
【基金】：潍坊市科技发展计划(2015GX077)~~
【分类号】：S823

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